MES Buffer Receipe - (Dec/27/2004 )

Does any one have the receipe for MES buffer used for protein separation on acrylamide gels?

Thanks in advance

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1x MES running buffer (50 mM Tris base, 50 mM 3–(N–Morpholino)propanesulfonic acid, 1 mM EDTA, 0.01% SDS at pH 7.3)

The stock is usually 20x and pH 7.2

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should store the stock at 4° and protected against light (MOPS...)

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