Protocol Online logo
Top : Forum Archives: : General Lab Techniques

Role of common compounds in different buffers and solutions - compounds are acting in solution as... (Feb/14/2006 )

Pages: 1 2 Next

ok i've sum up links to some of the common buffers or compounds present in solutios. As many of newbies ask what is the role of X and sometimes for the 4th time, relevant links may be found here (not all links are there, possibilities of missing ones ):

extraction of proteins, so Hepes, nacl edta glycerol and np40
B mercapto ethanol in cell culture
PBS in cell culture
role of DTT
role of DTT (2)
folate and DNA methylation
sodium azide
binding buffer(sodiumazide,glycine&nacl) // elution buffer (sodiumazide&citricacid(anhydrous&trisodiumsalt) // elutionbuffer (glycine&sodiumazide)
Poly (dI-dC)
Beta mercaptoethanol in a transformation reaction
sodium acetate in nucleic acids precipitation
restriction buffer and TAE
glycine in Ab purification
5%PEG in ligation
gelatin / BSA in PCR buffer
na deoxycholate
LB and SOC agar
NP 40 vs Triton X-100
role of polyadenosine in DNA extractions
what is TE buffer for?

NEW ONE (august):
tris and glycine in transfert buffer


QUOTE (fred_33 @ Feb 14 2006, 03:24 PM)

klick me, klick me!!
blink.gif biggrin.gif biggrin.gif


i've deleted the hfrom http and that drive a redirection to tpp... huh.gif huh.gif
i've edited your post too in order to fix the link.


Aught to make that a sticky Fred.


QUOTE (pBluescript @ Feb 16 2006, 02:52 AM)
Aught to make that a sticky Fred.

unsure.gif unsure.gif i must confess i don't understand this expression.... sad.gif
Do you mind to explain (or translate in french wink.gif ) ?
does that min : "better pin the topic"?


My guess of translation: make this topic "sticky"?


I guessed the reference is regarding a post-it (or 'sticky') note

as in, make a sticky note, or 'bookmark the post' to have it at your fingertips, hey?


At another forum I visit, the pinned topics are called "sticky" topics


thanks... I definitely go to US or england to get these nicknames and all "usual language".... unsure.gif


thanx alot fred biggrin.gif


Pages: 1 2 Next