lysis buffer - (Oct/27/2005 )
why are two different lysis buffers used for the lysis of RBCs and WBCs..what is the significance in using the two??
plze also give the composition of wbc lysis buffer
i agre with sasu.
But maybe the difference come from the fact that WBC have a nucleus and RBC don't
Red Blood Cells are much more osmotically sensitive and will therefore lysis before WBC. Many people use this to remove RBC from a lymphocyte prep.