Anode/Cathode buffer in Tris-Tricine SDS-PAGE - purpose of seperate buffers in anode and cathode (Jun/19/2008 )
Hi there everyone,
I have a relatively simple question. I'm trying to figure out why we use a seperate anode and cathode buffer for Tris-Tricine SDS-PAGE gels but not when running say a Tris-glycine SDS-PAGE gel. Both are discontinuous buffer systems, they use tris and tricine or tris and glycine so why does using tricine i assume force us to use differnet anode and cathode buffers?
Thanks!
I have a relatively simple question. I'm trying to figure out why we use a seperate anode and cathode buffer for Tris-Tricine SDS-PAGE gels but not when running say a Tris-glycine SDS-PAGE gel. Both are discontinuous buffer systems, they use tris and tricine or tris and glycine so why does using tricine i assume force us to use differnet anode and cathode buffers?
Thanks!
I don't have a ready answer, but I request that you research these search results, and if you do find an answer, kindly write it here for the future generation!
http://search.vadlo.com/b/q?sn=158621799&a...PAGE+&rel=0
..
there are a number of reasons to use different or the same buffer in the anode and cathode. these include cost, past practice, run time, etc.
having different pH in the anode and cathode affects the electric potential. current will be different at the same voltage than with the same buffer in both. this will make a difference to the speed and resolution of the gel run.
there are a lot of gel buffer formulations in use (or formerly in use). some use the same buffer, some use different buffers. they all serve(d) the purpose of the investigators using them.