Na-K phosphate buffer recipe? - (Jan/28/2008 )
I have an enzyme assay proctol that calls for 0.5M Na-K phosphate buffer. Does anyone know how to make this or have a protocol? I have made Sodium phosphate buffer and Potassium phosphate buffer, but never Sodium Potassium phosphate buffer. Any help would be greatly appreciated.
-garciapp-
prepare it the same way you would with sodium phosphate, but switch one of the solutions with the potassium salt (i would use the monobasic potassium phosphate to keep a higher sodium concentration).
-mdfenko-
QUOTE (mdfenko @ Jan 28 2008, 02:41 PM)
prepare it the same way you would with sodium phosphate, but switch one of the solutions with the potassium salt (i would use the monobasic potassium phosphate to keep a higher sodium concentration).
Thanks, that sounds easy enough. I just have never come across this buffer before.
-garciapp-