Protocol Online logo
Top : Forum Archives: : Molecular Biology

electrophoresis buffer - when I should replace running buffer (Aug/12/2002 )

I used TBE for running electrophoresis. I not sure when I should replace the buffer. Every time when I run?


Changing your buffer everytime is not necessary. Buffers need changing (in my opinion) when they become somewhat opaque or  have a high resistance to the current.