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Sodium phosphate buffer - (Jan/02/2009 )

Dear all,
What is the correct proportion to use Sodium phosphate buffer? if my culture broth is 100ml, how much of buffer i need to use? looking forward for your answers.. FYI this is for bradford assay and i m extracting protein from fungi. Thanks in advance.. smile.gif


i can't speak specifically about culture medium but we usually use phosphate buffer at 10-20mM (most of the time, 10mM). phosphate has a high buffering capacity.