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Difference between TE buffer at pH 8.0 and 7.5 - (Feb/02/2005 )

Hi all,

May anyone tell me the purpose of using different pH, at 8.0 and 7.5, of TE buffers for DNA and RNA disolution, respectively? Can TE at pH 8,0 be used for both DNA and RNA?

Thank you in advance.


The use of TE with slightly different pH values is based on early 80's literature that DNases are supposed to be less active at pH 8.0 and RNases less active at pH 7.5.
You can use pH 8.0 for both DNA and RNA.


Hi tfitzwater,

Thank you for the infos.