8-MOP solution - (Oct/16/2005 )
Has anyone know about 8-MOP solution? What is the purpose of adding this into PCR reaction?
here are few informations. 8-MOP is for 8-methoxypsoralen, also used for vitiligo treatment.
in bioforum, at this web page, i've found this :
If all else fails this may be an option; a reference in which it has been reported to have helped with a particularly sensitive protocol is Biologicals (2004) 32:183-193.
Hope that's useful for the really sensitive protocols,
this article says that at concentrations greater than or equal to 50 µg/ml, 8-MOP was found to inhibit the PCR. A concentration of 25 µg/ml was not inhibitory. Moreover, 8-MOP should be dissolved in DMSO and that a concentration
hope that helps you .