Ultrafiltration of Enzyme Solution? - (May/10/2007 )

Dear friends
Do you have any information or full text regarding the ultrafiltration of enzyme solutions or bacterial supernatant? As you know, in every stage of concentration or purification methos, there will be a loss in enzyme activity. but I have not found a significant text for ultrafiltration of enzyme(such as alpha amylase) which has been worked on this specific point of view.[color="#0000FF"][/color]

Thanks in advance

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loss of enzyme activity during UF often results from the long incubation time even at 4°C, and inactivation oenzyme by binding the filter membrane or the filter tube; if your enzyme is instabil or at very low conc, I would not recommend UF

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thank you very much for your explanation. As a procedure, I used centrifugal ultrafiltration, let the supernatant become 1/3 of the initiate one. the relative activity has not chenged significantly. but I have not a refference to compare my result with. what is your opinion?
best regards

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ok, I initially thought of high-pressure ultrafiltration; centriprep-like concentration of proteins is widely accepted and often used; so there is no special need of extensive citation;

if you describe in detail what you have done, and it works, you do not have to cite others, beside you think you have established a novel protocol or like to compare your results with others using similar or different protocols;

so you really have to check novelty by comparison with others...

if you like to quote, go to the manufacturer´s homepage for literature, or try to find some hits at PubMed

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?DB=pubmed

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Thank you very much Bearer, Yes you exactly got the point. Now I am preparing my paper. If I have more question, I will ask later. again thanks.

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