How to sterilize Ovalbumin solution? - (Jul/20/2008 )
I wanna sterilize high concentration ovalbumin solution. I hv tried to filtrate it...... but it block the filter. Since I will do the cell test, I cannot add sodium azide. So I think about UV.. Did anyone try it, Will it denature the protein? Is there any alternative sterilizing method?
I belive you have run out of options.
The only way around this would be if you need your final concentration to be lower than your stock solution. You can dilute and filter at that moment. If this is not possible, I would find out if there is another use for the ovoalbumin solution, freeze it and start over (I know, $$). This time: Open the flask under the hood (you need a brand new flask) and dissolve in a sterile solution.
UV will not sterilize it unless you were to spread it really thin on a petri dish and leave it for a long time, but, would you trust the final solution that results from this? (concentration wise AND the presence of free radicals, not to mention the probable structural changes to ovoalbumin).
Cut your loses.
Best regards, Alejandro
Why do you want to sterilize it? What kind of quality do you need? When it regards to endotoxin contamination I want to make you attentive that EndoGrade Ovalbumin is commercially available (endotoxin < 1 EU/mg) from Profos AG.
Why not filter it at a lower conc that doesn't clog the filter, then concentrate it? Does it need to be concentrated?