Developing solution at northern blot - (Nov/12/2005 )
At the last step of northern blotting, i mean at developing film step.
Sometimes i got black background at my film. and also sometimes i didn't get anything( developing solution not working.)
My procedures are, expeosed film 2 min in developing solution then rinse in water , 2 min in fixer solution. rinse in water.
But, i would like to say that i washed the tray of developing solution before using.
So, please if anyone knows the solution , i apperciate.
don't get that answer bad... but do as us.
prepare your own batch and do it handly as we do in our lab...