What is the difference between 2x and 4x sample buffer? - SDS Sample buffer (Mar/01/2002 )
I'm running SDS gels, I want to make up a 4x sample buffer. Do I just make up the 2x recipe in half the final volume?
here's a recipe for 4x from gibco
4x Sample buffer for (non-reduced gel): (for 10 mls use)
12% SDS 1.2g
40% Glycerol 4.0 ml
0.2 M tris-HCl pH7 2.0 ml of 1 M Stock
0.004% Bromophenol Blue 0.4 mg
4x Sample Buffer (for reduced gel):
Same volumes and amounts as #1 above but add to
solution 0.5 ml 2-Mercaptoethanol.