Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

Coating buffer for ELISA - Buffer composition needed...Help (Mar/02/2008 )


I need to make 0.05M carbonate-bicarbonate buffer (pH=9.6) for coating antibody in ELISA. Can somebody tell me the composition (including the volumes of the individual buffer) for preparing 100 ml of coating buffer. I will be glad if you can suggest me any useful web link for finding buffer compositions.

Thank You.


Here are some useful links about the coating buffer, hope this helps,



Hi tulip,

Thanks for the links. It helped. smile.gif


QUOTE (tulip @ Mar 3 2008, 10:53 AM)