Good buffer needed! - (Feb/17/2005 )
Small question for protein people. I want to use a nontoxic buffer with pH 5-6 to my proteins, which buffer you recommend?
Could just PBS be used or something?
Depends on what you want to do... adjusted PBS or HBSS would seem fine to me... or some Tris based buffer.
Can Tris based buffer be adjusted to pH 5~6?
I frankly don't know!?!? The more acidic Tris solution we use is 6.8...
tris cannot be used at ph less than 7.5. its buffering capacity diminishes below that pH. It wont buffer at all in the range 5-6. The buffer capacity of can system depend on the pka of the compounds used. for tris it is 8.3
National Chemical Laboratory
PBS is fine I guess
A good choice for pH 5-6 is citrate-phospahte buffer
If it is 5, i suggest acetate buffer
If it is 6, i suggest phosphate buffer