How to prepare buffers - (Oct/29/2008 )
I need a few hints on how to prepare a phosphate buffer
Details
Total phosphate concentration = 0.025 mol/L
pH =3
-University_Of_Toronto-
Strange buffer, never seen
Take 0.025 M/l fosforous acid and adjust the pH with NaOH 0.1 M
-Gerard-
QUOTE (Gerard @ Oct 29 2008, 06:42 AM)
Strange buffer, never seen
Take 0.025 M/l fosforous acid and adjust the pH with NaOH 0.1 M
Take 0.025 M/l fosforous acid and adjust the pH with NaOH 0.1 M
Why Strange? You have never worked with HPLC I assumed. Ever heard of HH equation
pH=pKa +Log [A-]/[HA]
where [A-] +[HA] =0.025 ?
-University_Of_Toronto-
It is strange because in real research, we seldom use phosphate buffer at pH3.0.
-Minnie Mouse-