problems with phosphate buffer in hydroxyl apatite chromatography - (Jun/04/2007 )
I am using 0.5M sodium phosphate buffer to purify proteasome using hydroxyl apatite chromatography. I do the purification in 4deg. I am constantly having problems with this buffer which crystallizes after standing for a while in the cold cabinet. I allowed the crystals to go into solution after bringing to room temperature. But the problem continues as i made a fresh buffer (titrating monobasic and dibasic).
Can somebody help me out?
we use potassium phosphate for our hydroxylapatite columns. it doesn't crystallize at cold room temperatures like sodium phosphate does.