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Dilution Buffer for Elisa - Dilution buffer for titre check of polyclonal serum or for monoclonal (Jan/10/2009 )

I want to do a titre check for polyclonal serum containing antibodies or for Serum containing monoclonal antibodies.
which will be the best dilution buffer for serum ?Waiting for a response


I usually dilute sera that I am testing in blocking buffer


PBS works fine most of the time for serum. Depending a bit on the blocking buffer composition, that should work fine as well. As long as you retain neutral pH and proper osmolarity, you should be fine.