Storage of B glycerophosphate NaF , Na3VO4, Na4P2O7 stock solution for lysis buf - How to store the protease kinase inhibitor soluble stock for lysis buf (Aug/08/2007 )

Dear all,

I wonder if the following solution have to be stored in a special manner. I was storing the solution stock at 4oC. But my western blot worked very well the two weeks after I did the solution, now (some month after) I only can see a very small band. It looks like I have some high degradation and I wonder if the storage of my stock solution for the lysis buffer can be responsible?

so I would like to know how to store:

0.5M NaF
100mM b-glycerophosphate
0.1M Na2P2O4
50mM Na3VO4(I allready read in the litterature that this may be stored at -80, but what about the rest?)

Thanks very much for your answer

cuca

I store all of these solutions aliquoted at -20°C and just put that much out, what is needed for the experiment. This works very well for me. I think 4°C may be to warm. -80°C is also possible.

i prepare not too much solutions and aliquot them at -20°.