WAHT IS TE BUFFER FOR? - (Jul/17/2006 )
WAHT IS TE BUFFER FOR???
TE stands for Tris EDTA. This is the buffer used for keeping DNA.
Agree with dnafactory
Yep, it's just a low strength buffer used in particular for eluting DNA from cartridges and columns. The low ionic strength and neutral pH cause the DNA molecules to dissociate from the resin/matrix. Read the QIAGEN manual for a better explanation.
as said above its a buffer (usually neutral pH) made up of tris and EDTA
Tris is used as the the buffer reagent, while EDTA is an interchelating agent, thus it basically takes up free ions form the solution, the reason for this is that most nucleases make use of ions to catlasyse their nuclease ability (something you dont want happeing in a buffer where you are storing your DNA!)
Tris is used as the the buffer reagent, while EDTA is an interchelating agent, thus it basically takes up free ions form the solution, the reason for this is that most nucleases make use of ions to catlasyse their nuclease ability (something you dont want happeing in a buffer where you are storing your DNA!)
great tip thankx
I would like to add that DNA (being an acid, after all) dissolves better at slightly alkaline pH; I use pH 7.5 to pH 8.0.
Additionally, the EDTA is (in my hands) optional -- in these days of kit-based plasmid extraction, the DNA recovered is usually free of nucleases, thus the EDTA performs no function, except to (perhaps) interfer with downstream reactions (like restriction digests).
Yes, I know -- the amount of EDTA delivered to a digest will ultimately be diluted in the volume of the digestion, and EDTA can guard against nuclease contamination from other sources, but I've always left it out without difficulty, preferring to store my DNA in just 5-10 mM Tris (pH 8.0) or sterile water.
ok.. understood. thx for the help
Additionally, the EDTA is (in my hands) optional -- in these days of kit-based plasmid extraction, the DNA recovered is usually free of nucleases, thus the EDTA performs no function, except to (perhaps) interfer with downstream reactions (like restriction digests).
Yes, I know -- the amount of EDTA delivered to a digest will ultimately be diluted in the volume of the digestion, and EDTA can guard against nuclease contamination from other sources, but I've always left it out without difficulty, preferring to store my DNA in just 5-10 mM Tris (pH 8.0) or sterile water.
I've read some researcher use concentration of 1/10 EDTa or 0.1mM. Sterile water pH can be decrease to 5-5.3 when gas CO2 is dissolve in it, is it not? Will that disrupt DNa?
yup. that is where the Tris buffer comes in.