buffer components - (Sep/02/2007 )
Please can anyone tell me what the purpose of the following buffer components are? I want to set up a transport assay, measuring intracullar pH changes using a dye. Just really curious as to what the components are for.. please help!!
140 mM NaCl, 4 mM KCl, 0.2 mM CaCl2, 1 mM MgCl2, 10 mM HEPES, and 10 mM D-glucose, pH 7.4
well it seems it's a buffer which mimics extracellular medium. High Na + low K+... hepes for buffering and necssity of glucose for isoosmolarity.
the salts are charged with positive and negative ions which affect transportation