DEPC before or after solution preparation?... - (Apr/21/2006 )

quite a basical question...
I want to prepare a buffer for RNA.
it's a na2hpo4 / nah2po4.
I was wonddring : can i treat the solutions with DEPC or should i depc treat the water before using the water for my buffers?....

See this Ambion Technical Note on DEPC: http://www.ambion.com/techlib/tb/tb_178.html

HI,
i would prefer to prepare the buffer first and treat with DEPC.
because i will be sure of my DEPC treatment...

hi
thanks payeli.
i've done like you describe. (in fact it's your post and in order to be sure that the vessel is also clean which decides me to do that.
thanks.

hi member
in my knowledge the DEPC compoud affect only the tris buffer especially reacts with the amino groups and impeds its buffering action