sterilization of buffer - (Dec/22/2006 )

hi
plz solve my querries here

1.can we autoclave phosphate buffer, cobalt chloride and biotin solution??
2. how to detect nicotinamide form your reaction solution other than HPLC and IR method??

Thank u
Nalinik

-nalinik-

i would not autoclave biotin, but phosphate buffer is ok

-fred_33-

QUOTE (nalinik @ Dec 22 2006, 06:10 PM)

Q1: Pi buffer, CoCl2: yes; biotin: no

Q2: ever tried thin layer chromatography with fluorescence detection or a specific staining method you may know

-The Bearer-

hi
thank u

-nalinik-

i dont sterilize phosphate buffer

-T. reesei-