Azacytidine mother solution - (Mar/07/2005 )
I’m starting to work whit Azacytidina. To prepare a mother solution I dissolve 24mg on 20uL of wather: acetic acid (1:1) as SIGMA says I but the aza not dissolve. Then I’m going to add PBS to obtain a 10mM mother solution.
I would appreciate if somebody could help me to dissolve the Aza!
Thanks in advance
Do you mean azacytindine stock solution? check this discussion
Hi again Kawaka,
I think your mother solution is too concentrated, 25mg in 20uL? You can dissolve in 1:1 H2O/acetic acid to a final conc of 10mM, but once you add it to your culture media, the pH significantly decreases thus affecting your cells, I would take the suggestion from the link mentioned above and dissolve in PBS. it works!
i am pretty sure azaC is unstable in solution. Read the Sigma product sheet. It is available online. It would be wise, even if painful to weigh out a little quantity and make a fresh stock everytime.
you are absolutely correct eagertolearn. azaC has a very short half life and if possible you should use freshly made up stocks each time.
does anyone maybe know the half-life of 5-AzadCytd?