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Electrophoresis Related Discussions

1. SDS Gel for 2D -DIGE - Problems having SDS Page to polymerize (reply: 3)
   
2. how to distinguish supercoiled DNA from linear one in agarose gel - (reply: 2)
   
3. slow electrophoresis, somebody can help me? - troubles in electrophoresis (reply: 4)
   
4. Capture of silver stained gels - (reply: 1)
   
5. Agrose gel - (reply: 4)
   
6. Direct immunodetection within SDS-PAGE GEl? - Is it possible? (reply: 5)
   
7. SDS in gel or not? - (reply: 2)
   
8. How to rehydrate a SDS-PAGE gel - (reply: 2)
   
9. DNA Gel - (reply: 6)
   
10. Protein size on SDS gel - (reply: 1)
    
11. Repairing wire in gel apparatus - (reply: 3)
    
12. can I autoclave agarose? - (reply: 3)
    
13. Difference between TBE- and Denaturing Agarose Gel Electrophoresis of RNA - Gel for RNA (reply: 2)
    
14. Staining phosphoproteins on the SDS gel? - Can Pro-Q Diamond be used for a crude sample? (reply: 1)
    
15. Electrophoresis RNA - (reply: 1)
    
16. 3.5% acrylamide gels and transfer - I need tips (reply: 4)
    
17. This is the gel pic that is confusing me and my boss - (reply: 7)
    
18. Stacking gel necessary? - (reply: 5)
    
19. gold view and sybr safe gel stain - any used gold view gel stain (reply: 2)
    
20. Silver Stain 2D gels - Trouble with silver staining 2D gels (reply: 1)
    
21. Gel extraction kit...which one is the best? Cheapest? Coolest? lol - (reply: 4)
    
22. Sterilization technique for gel - (reply: 3)
    
23. Non-denaturing western blot - I need a transfer protocol for non-denaturing gel (reply: 1)
    
24. Gel filtration - problem! (reply: 8)
    
25. MASCOT results vs 2D gel image : Which one do you trust? - Protein positive hits in MASCOT do not agree with a spot's position!! (reply: 2)
    
26. Improved electrophoresis of nucleic acids. - (reply: 15)
    
27. gel purifications of DNA fragm > 10Kb - (reply: 9)
    
28. Transformants pictures in gel - (reply: 2)
    
29. oxidized vs native protein? diffeerence expected on Isoelectric focusing gel? - why? (reply: 2)
    
30. Problems with zymography - Running SDS page gels protein wont migrate down the gel (reply: 4)
    
31. plasmid digestion gel - (reply: 4)
    
32. agarose gel electrophoresis - Question (reply: 3)
    
33. how much agarose to use - (reply: 5)
    
34. Extraction of PCR product from gel - (reply: 4)
    
35. gel and monoclonal antibody issues - (reply: 3)
    
36. How to extract Large protein from Low percentage SDS-Glycine-PAGE gel? - (reply: 1)
    
37. Native PAGE gel - (reply: 3)
    
38. Can we store the gel slice after cutting out the band and before gel extraction? - (reply: 3)
    
39. 10% acrylamide/bisacrylamide, or 15% ? - SDS PAGE gel making (reply: 2)
    
40. Gel doc camera - Resolution (reply: 6)
    
41. 2D gels.. comparison software? - (reply: 1)
    
42. 2D Electrophoresis for protein inclusion bodies/lysate? - sample preparation? (reply: 1)
    
43. DNA gel electrophoresis problem? - (reply: 5)
    
44. DNA gel to Test Ligation? - (reply: 2)
    
45. Problem with drying down polyacrylamide gel - (reply: 4)
    
46. Frozen Protein A agarose beads - (reply: 1)
    
47. what could cause gel not to polymerize (solidify)? - gel making (reply: 8)
    
48. Need to sequence gel purified vectors exposed to UV? - (reply: 2)
    
49. poor electrophoretic protein transfer - (reply: 5)
    
50. RNA gel loading buffer? - (reply: 5)
    
51. Check the ligation product, but got two fragments with strange sizes on the gel - (reply: 7)
    
52. Sample (protein) recovery on 2D gel - (reply: 1)
    
53. multiple gel extractions - (reply: 5)
    
54. GEL Extraction (any advanced techniques ?) - (reply: 7)
    
55. Problems with bubbles in collagen gels - (reply: 1)
    
56. DNA gel problem - (reply: 13)
    
57. combining the results from different gels in western blot - (reply: 3)
    
58. Is it possible to use a PCR cleaning kit to clean a band from a TAE-Agarose gel? - (reply: 1)
    
59. transfering proteins from 2-D gel to nitrocellulose membrane - (reply: 5)
    
60. Great signal but nothing on gel - (reply: 3)
    
61. Dna purification from gel - (reply: 20)
    
62. Normal agarose gel vs formaldehyde agarose gel - (reply: 1)
    
63. New to RNA... Does this cDNA gel look OK? - (reply: 2)
    
64. Screening Tranformation Colonies: Funny Gel Pic and no plasmid?!?! - (reply: 9)
    
65. no high molecular weight bands seen.. know to be present.. - where did they go.. in a gradeint gel? (reply: 3)
    
66. 2-D gel analysis - (reply: 3)
    
67. Homebrew Gel Documentation System - Burnout of Digital Camera? - (reply: 9)
    
68. Strange large bands on my PCR gel, has anyone seen this before? - PCR (reply: 3)
    
69. gel extraction - (reply: 1)
    
70. Can gel filtration effect buffer composition? - (reply: 4)
    
71. Plasmid DNA preparation - two DNA bands in gel (reply: 5)
    
72. Help troubleshoot SDS gel - smear down well - (reply: 2)
    
73. High concentration DNA on spectrophotometer but no band on agarose gel. WHere is - DNA from bone and teeth (reply: 5)
    
74. Coomassie Dye - SDS-PAGE gel - (reply: 2)
    
75. How to make a neat gel for master thesis? - Expressed protein on SDS-PAGe gel (reply: 3)
    
76. Proper ratio of stacking and separating gel - (reply: 3)
    
77. RNA denatruring agarose gel electrophoresis- help on sample preperation - (reply: 4)
    
78. benefits of urea in SDS PAGE gel - (reply: 9)
    
79. lowest concentration of RNA - to be visible on gel (reply: 3)
    
80. dna ladder seperation in gel - (reply: 4)
    
81. western blot -- running the gel - (reply: 6)
    
82. using piece agarose band directly on PCR - does it work? - (reply: 3)
    
83. what is more important in protein electrophoresis? voltage or current? - sds-page (reply: 10)
    
84. Silica Gel Chromatography beginners questions - Need some help getting started... (reply: 1)
    
85. Please help me with my sonication (gel included)... - I know, everyone asks the same thing, but I'm desperate (reply: 20)
    
86. Help! low molecule weight peptides electrophoresis! - (reply: 2)
    
87. How much RNA at least on agarose gel? - (reply: 3)
    
88. Native PAGE problem - Protein doesn't enter in resolving gel (reply: 1)
    
89. DNA spin column problems - Possible protein contamination and blank gel (reply: 6)
    
90. Denaturing Gels and non denaturing gels (Gel electrophoresis) - Gel electrophoresis (reply: 2)
    
91. Looking for perfect acrylamide gel EtBr stain - (reply: 4)
    
92. Native gels for proteasome activity assay - (reply: 3)
    
93. Gel destaining - (reply: 1)
    
94. no bands, no ladders, nothing at all in my gels - (reply: 7)
    
95. silly question...maximum loading for 1.5mm gel - (reply: 9)
    
96. reuse of mineral oil in 2 D gel electrophoresis - can we reuse mineral oil for 2 D gel electrophoresis (reply: 1)
    
97. Affect of pI on protein migration on SDS-PAGE gel - (reply: 5)
    
98. PGEM-T easy gel - partial digestion? - (reply: 2)
    
99. Running different % acyrlamide gels - (reply: 3)
    
100. Gel purification of large plasmids for PCR or sequencing, HELP! - (reply: 3)
     
101. running a 6% SDS gel - (reply: 1)
     
102. Removing bubbles from collagen gel - (reply: 2)
     
103. Polyacrylamide gels shriveling up during transfer - (reply: 1)
     
104. gel percentage & loading control for ATM and ATR? - western blotting (reply: 6)
     
105. linearized vector purification from TAE agarose gel - (reply: 5)
     
106. EC invasion into collagen gels and serum concentration - (reply: 3)
     
107. Western blotting conditioned media problem - Media will not separate when run on gel (reply: 2)
     
108. Current for gel electrophoresis - Fried my DNA I think (reply: 8)
     
109. How to improve the agarose gel? - (reply: 10)
     
110. problems with zymography gels - (reply: 1)
     
111. Use of silver staining for DNA in polyacrylamide gels... - or any other helpful suggestios to improve detection! (reply: 5)
     
112. Gravity flow gel filtration chromatography - (reply: 2)
     
113. Sephadex G-50 gel filtration chromatography - (reply: 6)
     
114. Sephadex G-50 gel filtration chromatography - (reply: 1)
     
115. native gel problem - (reply: 7)
     
116. SDS Gel - My protein bands go to the bottom of gel (reply: 9)
     
117. digested run slower on gel? - (reply: 6)
     
118. MoBio Plasmid Purification and Gel Extraction Kits - Problems with MoBio Kits (reply: 3)
     
119. 2 - DE Gel - Protein Transfer - (reply: 2)
     
120. DNA Footprinting Gel - (reply: 3)
     
121. pulsed field gel electrophoresis of genomic DNA - (reply: 1)
     
122. Taking Agarose Gel Pic with Regular Digital Camera - (reply: 14)
     
123. Agarose gel - (reply: 6)
     
124. 10kDa-20kDa proteins are missing on coomassie stained gel - (reply: 5)
     
125. sds page (gel preparation) - (reply: 4)
     
126. need help with my rna gel - (reply: 12)
     
127. Need help, what's wrong with my DNA gel? - (reply: 10)
     
128. gel bond and gel dryer - put gelbond in gel dyer (reply: 5)
     
129. Is there ay staining solution to stain colony on agarose gel - (reply: 2)
     
130. Ultra pure agarose - (reply: 4)
     
131. Question of QIAGEN's QIAquick Gel Extraction Kit - (reply: 10)
     
132. DNA gel purification without a spin column - (reply: 4)
     
133. DNA from Agarose gel basic questions - Basic functions of chemicals in DNA extraction from Agarose Gel (reply: 2)
     
134. Of voltage and bands - Agarose gel electrophoresis (reply: 3)
     
135. Genomic DNA smears on gel - (reply: 5)
     
136. RNA samples degraded in gel... where is the source of contamination? - (reply: 9)
     
137. basic dilution question for making gels - (reply: 1)
     
138. RNA gel - multiple bands - (reply: 2)
     
139. RNA Gels - (reply: 3)
     
140. Gel or column purify - (reply: 4)
     
141. Small DNA fragments, which electrophoresis shoudl i choose? - (reply: 10)
     
142. SOS: DNA purity problems - Low A260/230 of gel extracted DNA samples (reply: 4)
     
143. native gel problem: blurry bands - (reply: 11)
     
144. Native gel and salt - (reply: 2)
     
145. Qiagen gel extraction accident. - is all lost? (reply: 1)
     
146. Electrophoresis with sybrgreen - (reply: 5)
     
147. NuSieve GTG agarose gel takes forever to dissolve - (reply: 8)
     
148. What should 75 ug of total protein look like on gel? - Help - My 280 reading says I am loading 75 ug... (reply: 4)
     
149. Question about tricine gels - (reply: 1)
     
150. Tips on improving ligation+transformation from gel extraction - Let's contribute! (reply: 1)
     
151. gel compar II - (reply: 1)
     
152. Does phosphorylation affect protein migration in SDS-PAGE gel? - (reply: 9)
     
153. bumps in bottom part of sds gel - bumps in bottom part of sds gel (reply: 2)
     
154. Nano Drop measurements and RNa Gels incompatible? - (reply: 2)
     
155. protein-DNA interaction in gel filtration - (reply: 4)
     
156. gel ananlysis software - (reply: 6)
     
157. Agarose Gel instead of PAGE: Is it possible ? - (reply: 5)
     
158. gel formula - (reply: 1)
     
159. SDS gel image : why the band looks like 'stapler bullet' - (reply: 3)
     
160. Only 18S RNA Band shows up in RNA gel, why? - (reply: 2)
     
161. peptid conjugation to BSA ? No band in the gel? - (reply: 1)
     
162. Native acrylamid gel - (reply: 10)
     
163. Troubleshoot SB buffer electrophoresis problem - (reply: 6)
     
164. Protein A- agarose or protein A- sepharose? - immunoprecipitation (reply: 1)
     
165. Ratio of acrylamide to bis-acrylamide - different percentages for DNA and protein gels (reply: 1)
     
166. RNA gel extraction - (reply: 2)
     
167. PLasmid extraction: good nanodrop reading but NOTHING on the gel! - (reply: 4)
     
168. difference between Qiaex and Qiaquick gel extraction kits - (reply: 5)
     
169. RNA gel electrophoresis - multiple conformers or multiple products? - (reply: 1)
     
170. Cy3/Cy5 dyes and Urea denaturing PAGE gels - (reply: 1)
     
171. Gel extraction problem ~HELP~ - (reply: 3)
     
172. Acrylamide gels blurring DNA - (reply: 5)
     
173. High MW smear - how can I get all protein into the gel? - (reply: 3)
     
174. Does 20x TTE buffer REALLY expire? - polyacryamide gels; buffers (reply: 1)
     
175. plz guide me.i got many band in one well in agrose gel electrophoresis - PCR (reply: 8)
     
176. VERY SLOOOOOW running gels (SDS-PAGE) - (reply: 8)
     
177. Could you run Taqman PCR on gel to see the band? - (reply: 1)
     
178. extra bands on SDS PAGE gel - (reply: 4)
     
179. Gap between bands too small - DNA Gel Electrophoresis (reply: 6)
     
180. problems with RNA bands - on agarose gel (reply: 1)
     
181. problems with RNA bands - on agarose gel (reply: 2)
     
182. difference between "electrophoresis grade glycine" and regular old gly - (reply: 1)
     
183. Gel purification: SpinX vs commercial kits - Gel purification (reply: 3)
     
184. My DNA fragment in agarose gel is in 4 degree - Can I extract the fragment and use it for cloning??? (reply: 6)
     
185. Gel loading Question - (reply: 1)
     
186. Easy Question on seperating 1kb and 1.3kb gel bands - what percentage? (reply: 3)
     
187. electrophoresis for RNA - agarose gel or FA gel?? (reply: 9)
     
188. [western blotting] Loading control in 6% gel? - Please suggest good loading control protein of high molecular weight (reply: 2)
     
189. bands/dyes don't move on gel - (reply: 9)
     
190. shotgun cloning: gel shows inserts but only plasmid sequence obtained - (reply: 1)
     
191. Is there any good coomassie stain which is comparable to silver stain? - searching for a new staining method for 2D gels (reply: 6)
     
192. the problem in gel purification - (reply: 4)
     
193. How to choose the ready gel for western blotting - (reply: 3)
     
194. QIAEX II gel purification kit - (reply: 11)
     
195. RNA extraction/electrophoresis problem - (reply: 8)
     
196. need help, my collagen gels are watery - (reply: 1)
     
197. Why is resistance increasing with SDS PAGE gel run - (reply: 4)
     
198. polyacrylamide gel for DNA analysis - (reply: 4)
     
199. no band in 6% gel - (reply: 4)
     
200. Gel shift on formalin tissues? - EMSA (reply: 1)
     
201. Tris Tricine Gel Running Buffer - (reply: 1)
     
202. "Question"Why Gel Extraction Kit not working? - (reply: 6)
     
203. Freeze and crush method for gel extraction - (reply: 2)
     
204. Uneven band migration on agarose gel - (reply: 4)
     
205. band disappearance on agarose gel - (reply: 3)
     
206. TBE urea gel sample buffer - formamid or not? (reply: 2)
     
207. Formaldehyde Gel Electrophoresis on non-denatured/denatured RNA - No/Faint RNA bands after denatration (reply: 1)
     
208. Westerns from 3D collagen gels - (reply: 1)
     
209. recipe for SB buffer for electrophoresis - (reply: 4)
     
210. Gel without ethidium - (reply: 8)
     
211. sticky and blunt DNA on gel migration - (reply: 5)
     
212. Strange "V" band in Gel Shift - (reply: 4)
     
213. DNA yields with the Qiagen PCR gel purification kit - (reply: 8)
     
214. semi-dry transfer for midi gels - (reply: 4)
     
215. The top of PAGE gel is not polymerizing - (reply: 6)
     
216. gel elution problem - (reply: 1)
     
217. shaded bands in agarose gel - (reply: 8)
     
218. See small size difference on gel? - (reply: 2)
     
219. saving your gel for future - (reply: 3)
     
220. Weird two bands of my purified vector DNA apeared on my gel ? - (reply: 5)
     
221. 2D Gel Electrophoresis Software - (reply: 1)
     
222. help with LDH separation using agarose gel electrophoresis - (reply: 5)
     
223. After extracting total RNA from tissue using trizol reagent and phase lock gel,h - I am going to extract bothe total RNA and genome DNA from one sample (reply: 4)
     
224. SDS Gel - (reply: 4)
     
225. White background on DNA gels - (reply: 8)
     
226. Circular ligation product migration on gel? - (reply: 1)
     
227. Setting tris-glycine gels and storing them BEFORE running them? - (reply: 7)
     
228. RNA gel electrophoresis - any recommendations? - (reply: 12)
     
229. Why do I got 3 bands of RNA gel? - (reply: 3)
     
230. question about agarose gel - (reply: 7)
     
231. Gel Fixation - (reply: 6)
     
232. glycerol [c] for dna in gel - (reply: 5)
     
233. Problems after gel purification of plasmids - Restrictases and ligase do not work on gel purified DNA (reply: 2)
     
234. RT-PCR - necessary to run electrophoresis gels? - (reply: 4)
     
235. Bloting IEF gels onto PVDF membranes? - (reply: 1)
     
236. identifying a gel band by mass spectrometre? - problem in identifying the protein, no matches (reply: 13)
     
237. problem in separating gel - (reply: 6)
     
238. Gel purification of plasmid after each of these steps? - (reply: 1)
     
239. horizontal streaking in 2-D gel? - for help (reply: 4)
     
240. southern blot SSC bufffer doubt - do we need to use electrophoresis grade sodium citrate? (reply: 2)
     
241. Agarose gel solidifying - from asia-poland (reply: 6)
     
242. Lack of bands in SDS-PAGE gel - (reply: 4)
     
243. Gel Shifts: DIG labelling - (reply: 1)
     
244. DNA Extraction for Ladder Assay - Protocols to get best Agarose gels (reply: 4)
     
245. Electrophoresis problem - (reply: 9)
     
246. DGGE - PCR and gel conditions - (reply: 1)
     
247. SYBR Green Nucleic Acid Gel Stain - alternative for EtBr? (reply: 1)
     
248. Preparing Gel Loading Buffer - (reply: 6)
     
249. Agarose gel concentration for small and similar pcr pdts - (reply: 7)
     
250. fresh gel - (reply: 5)
     
251. do you subtract gel base intensity from PCR band intensity? - urgent require (reply: 3)
     
252. samples floating out of SDS-page gel well immediately after loading - (reply: 7)
     
253. Expect monomer on SDS PAGE gel get dimer or larger - (reply: 3)
     
254. How to dilute loading dye? (For gel electrophoresis) - (reply: 6)
     
255. Agarose 'rafts' blocking the top of sample well - Difficulty pouring a 3% gel (reply: 4)
     
256. Electrophoresis, pls urgent - (reply: 17)
     
257. Urea gels - (reply: 5)
     
258. Low concentration after gel purification - PCR product purification (reply: 6)
     
259. Free Agarose - Free Agarose (reply: 8)
     
260. Acrylamide gel with abnormal band - (reply: 4)
     
261. Elution of a smear from acrylamide gel - Electrophoresis and gel elution (reply: 2)
     
262. Two Tm peak in real-time but only one product on a gel? - What's goning on????? (reply: 4)
     
263. PCR and Digest cleanup before ligation. - A shortcut by avoiding gel purification. (reply: 15)
     
264. Gel purification of DNA bands-any alternates? - (reply: 4)
     
265. LDH isoenzyme separation by Electrophoresis - (reply: 2)
     
266. Column chromatography with silica gel - (reply: 2)
     
267. real-time PCR products on gel look good but melting curve is horribel - (reply: 2)
     
268. 2D gels: Is there a protein assay thiourea is compatible with? - proteomics (reply: 2)
     
269. unsuccessful gel purification - (reply: 8)
     
270. gel purification for PCR product - (reply: 5)
     
271. Acrylamide gel preparation - for SDS-PAGE (reply: 4)
     
272. Help! I need to run collagen on SDS gel - (reply: 2)
     
273. sample preparation for 2d gel elctrophoresis - (reply: 2)
     
274. PRotein marker can give concentration of my protein in gel? - (reply: 4)
     
275. Gel appeared during serum collection - (reply: 1)
     
276. Gel shift with high isoelectric point protein - (reply: 1)
     
277. Quantifying gel purification products - (reply: 12)
     
278. capilary electrophoresis - (reply: 2)
     
279. strange red lines in all of my agarose gels from PCR - (reply: 2)
     
280. Ethidium bromide gel - (reply: 15)
     
281. About gel exlusion chromatography - (reply: 5)
     
282. electrophoresis buffer become very hot - (reply: 11)
     
283. protein extraction form SDS PAGE gels - (reply: 4)
     
284. Tricine gel storage - can i store fully made gels overnight? (reply: 6)
     
285. Need denature (94oc in 5 mintute) for agarose gel? - (reply: 7)
     
286. agarose gel electrophoresis - problems in marker migration (reply: 14)
     
287. Making high concentration agarose gels - A request for advice (reply: 7)
     
288. Differences between DGGE & standard electrophoresis? - (reply: 1)
     
289. Dissociation curve vs Gel image - Which one to trust? (reply: 1)
     
290. PAGE gel DNA band shape - (reply: 4)
     
291. Is 20% methanol sufficient to fix protein in gel? - (reply: 5)
     
292. DNA extraction from gel - (reply: 16)
     
293. electrophoresis question - (reply: 6)
     
294. Smearing antibody and DTT - how to interpret the gel? - Aggregated antibody (reply: 1)
     
295. help with agarose gel problem - (reply: 3)
     
296. How to quantitate the pcr product from gel picturess - (reply: 4)
     
297. Gel Loading- Equal Protein or equal volumes? - (reply: 2)
     
298. How to elute protein from SDS-PAGE gels - (reply: 3)
     
299. Electrophoresis buffer prep questions - (reply: 9)
     
300. How to run poly-lysine on tris-tricine gel - (reply: 4)
     
301. Extract DNA from gel - (reply: 5)
     
302. Pulse Field Gel Electrophoresis - for E.faecium (reply: 4)
     
303. Ligation reaction gel picture - (reply: 1)
     
304. Recipe needed for native gel - (reply: 1)
     
305. Transfection of expression vector for gel shift - how much vector should be transfected? (reply: 2)
     
306. What safety and risk assessments should i be aware of in doing PCR and gel elect - (reply: 2)
     
307. contaminated gel eluted DNA fragment - (reply: 8)
     
308. Improvement to Lammli Gel electrophoresis - The occasional gem (reply: 2)
     
309. Loss of phosphorylation after gel purification? - (reply: 3)
     
310. rehydrate dried gel - (reply: 4)
     
311. Uneven background of DNA electrophoresis image - (reply: 4)
     
312. i have to put gel until tomorrow - URGENT (reply: 6)
     
313. polyacrylamide gel electophoresis - (reply: 8)
     
314. melting a polyacrylamide gel? urgent - (reply: 6)
     
315. low melting agarose - low melting agarose (reply: 3)
     
316. Electrophoresis of DNA - (reply: 7)
     
317. Gel extraction protocol for DNA purification - Any suggestion? (reply: 11)
     
318. Locate a lab for PCR and Gel work - (reply: 2)
     
319. Protein high molecular weight standards for gel filtration - (reply: 2)
     
320. dna recovery from gel - (reply: 7)
     
321. moving of gel - from gel plate (reply: 4)
     
322. agarose gel melted after 4h running.. - (reply: 11)
     
323. Visualising 200-400+ kDa proteins by gel electrophoresis - (reply: 3)
     
324. dna on gel image help - (reply: 3)
     
325. PCR DNA IMAGES ON GEL HELP WANTED - (reply: 3)
     
326. FPLC: Gel Filtraito - Running Standards, Triton X-100, Mitochondria on a column (reply: 3)
     
327. I need your help regarding HAEMOGLOBIN ELECTROPHORESIS - (reply: 1)
     
328. Buffer BPTE for DNA electrophoresis - (reply: 3)
     
329. Serious Mini Gel Stack Problems. - (reply: 16)
     
330. can agarose be removed by phenol? - (reply: 5)
     
331. My DNA shoots out of the wells in my Agarose Gel... why? - Agarose Gel Loading Help (reply: 14)
     
332. 2D protein gel for miRNA target screening? - (reply: 2)
     
333. running DNA on gel - (reply: 2)
     
334. low MW bands left on gel during transfer to PVDF - (reply: 1)
     
335. Agarose Gel Electrophoresis - Troubleshoot (reply: 12)
     
336. gel electrophoresis - problem with stacking (reply: 22)
     
337. large proteins don't analyse well-sds page gels - (reply: 12)
     
338. How long to store 1% agarose gels? - (reply: 11)
     
339. Simple Question... Volts/amps for running mini gels? - Which volts/amps are the best, mine do not work! (reply: 11)
     
340. Why high voltage is used in capillary electrophoresis? - (reply: 1)
     
341. making Ficoll gel loading buffer - (reply: 1)
     
342. What type of native gel (pH/acrylamide %) do I use for a protein with the pI=9.0 - (reply: 4)
     
343. extracting DNA frm agarose gel no result - (reply: 3)
     
344. Protein A agarose for CHIP--pre-treatment is needed? - (reply: 2)
     
345. How to take picture of dna electrophoresis without a gel documentation unit? - (reply: 3)
     
346. Blotting gel after Bio-Rad Coomassie staining? - (reply: 5)
     
347. Shadow in agarose gel - (reply: 8)
     
348. native gel? - want protocol (reply: 7)
     
349. HELP: Excision of gel band from EMSA gel - (reply: 1)
     
350. capillary electrophoresis - (reply: 1)
     
351. Help: ECM Extraction from cells grown in Alginate gels - (reply: 4)
     
352. Molecular weight determination by SDS page and gel filtration - (reply: 5)
     
353. DNA markers - Gel run agarose or PAGE (reply: 1)
     
354. Please check my ChIP sonication gel - (reply: 4)
     
355. destaining silver stained gels - (reply: 1)
     
356. Gel Electrophoresis & Staining - (reply: 2)
     
357. Help Reading Sonication Gels! - (reply: 4)
     
358. Frowns in polyacrylamide gel - (reply: 8)
     
359. useful tips for low melting temperature gel purification. - please share your experiences. (reply: 2)
     
360. high sizes on gel - (reply: 5)
     
361. running a gel with primers - (reply: 2)
     
362. Acrylamide/Agarose gels on nucleic acids... - Differential use...? (reply: 1)
     
363. ethanol precipitation before gel purification... - (reply: 5)
     
364. Questions about protein gel loading and immunoprecipitation - (reply: 4)
     
365. In gel electrophoresis, what are the effects of sample loading on band size? - (reply: 3)
     
366. What to load in empty well on SDS gel? - (reply: 8)
     
367. why protein gels are run at constant current and constant voltage? - (reply: 2)
     
368. Protein purification for rabbit injection - SDS-PAGE gel, excise band, inject into rabbit?? (reply: 5)
     
369. what speed to centrifuge down agarose bead? - (reply: 4)
     
370. proteomic study at 1-D and 2-D electrophoresis. - wants your opinion ! (reply: 5)
     
371. Uncut genomic DNA - Migrate through the gel (reply: 3)
     
372. analysis of DNA using BamH1 and agarose gel electrophoresis - interpretation of results! (reply: 5)
     
373. protein bands are not migrating over to the whole gels. - please suggest me something, very urgent ! (reply: 8)
     
374. Gel Electrophoresis Bowed Migration Pattern Troubleshooting - (reply: 3)
     
375. Problem with agarose gel? or with RNA ? - (reply: 7)
     
376. How can I dissolve 6%polyacrylamide gel? - (reply: 2)
     
377. In gel electrophoresis... - (reply: 2)
     
378. gel keeping for Mass Spec - (reply: 5)
     
379. What factors affect the separation and visualisation of DNA fragments in gel ele - (reply: 1)
     
380. can someone tell me about extraction DNA from polyacrylamide gel? - (reply: 10)
     
381. why not boiled before electrophoresis? - (reply: 9)
     
382. streaking in 2D gels - (reply: 2)
     
383. Does anyone add Etbr to running buffer used to make agarose gels? - (reply: 14)
     
384. Viral RNA - have you ever run it in a gel? (reply: 6)
     
385. Lab velocity - free samples for gels and UV cuvettes (reply: 5)
     
386. In DNA gel electrophoresis, what happens to lambda fragments after being treated - (reply: 2)
     
387. 3D collagen matrice - Polymerization of collagen gel (reply: 5)
     
388. RT-PCR product dilution and electrophoresis - (reply: 2)
     
389. How to calculate DNA concentration after PCR manually - not using spectrophotometry or electrophoresis (reply: 6)
     
390. Qiagen gel extraction kit - strange buffer color (reply: 7)
     
391. Protein extraction from SDS gel - (reply: 6)
     
392. weak band during denature agarose gel electrophoresis of RNA - RNA denature agarose gel electrophoresis (reply: 3)
     
393. Low yield after gel extraction - (reply: 12)
     
394. Agaroase gel analysis of ligation reaction - (reply: 6)
     
395. Problem loading SDS-PAGE gel - (reply: 19)
     
396. Strange gel pattern, is EtBr the cause? - (reply: 1)
     
397. Help: Can't see any positive staining at all... SDS PAGE gel - (reply: 5)
     
398. basic gel question - (reply: 7)
     
399. Gel Shifts: Non-Specific Competition - (reply: 1)
     
400. advice on A/G agarose for ChIP - which gives the lowest background (reply: 3)
     
401. need advice on qPCR results (see attachment) - melting curve - gel picture (reply: 5)
     
402. Smear on gel after MiniPrep - Is this RNA? (reply: 10)
     
403. analysing gel bands - analysing gel bands (reply: 1)
     
404. RNA migration in agarose formaldehyde gel - (reply: 6)
     
405. Smeary DNA band on agarose gel - (reply: 5)
     
406. difference between RNA & cDNA gel image - (reply: 1)
     
407. Gel purification of PCR products - (reply: 2)
     
408. S-500 Gel filtration - molecular weight calibration (reply: 1)
     
409. how to run native PAGE gel? - (reply: 3)
     
410. Gel filtration estimations - (reply: 1)
     
411. SDS page gel GST beads or not to? - (reply: 3)
     
412. Expected bands in agarose gel electrophoresis - (reply: 3)
     
413. running itme for gels - (reply: 3)
     
414. HELP! PAGE Gel Running problem - (reply: 9)
     
415. DNA gel picture labeling - too many samples to label by hand - (reply: 2)
     
416. Native Gel bovine IgG - Native Gel (reply: 8)
     
417. Fuzzy bands on agarose gel after ligation - (reply: 7)
     
418. SDS-PAGE gel interpretation - sds page gel band reading (reply: 3)
     
419. phenol/CHCl3 vs purification from gel - (reply: 1)
     
420. PROTEIN AND DNA GEL ELECTROPHORESIS - GEL ELECTROPHORESIS (reply: 4)
     
421. protease bands on the gel.... - should they be visible? (reply: 11)
     
422. Single Colony Gels? - (reply: 7)
     
423. Gel and Ct-values relationship: is there a probe problem? - Bands on gel don't fit my expectations... (reply: 2)
     
424. Protein gel Loading troubles - Protein prep too viscous (reply: 4)
     
425. electrophoresis DNA yeast - (reply: 3)
     
426. Gel shift - (reply: 1)
     
427. differential electrophoretic migration of phosphorylated proteins - (reply: 2)
     
428. Screening for a 30bp difference using an agarose gel. - (reply: 4)
     
429. can protein A agarose precipitate anti goat IgG? - (reply: 2)
     
430. nucleic acids gel densitometry - (reply: 1)
     
431. DNA quantitation on agarose gel - (reply: 11)
     
432. Absence of bands in some lanes of gel electrophoresis - What are the reasons? (reply: 2)
     
433. Help: How to run Histone on SDS-PAGE gel? - (reply: 6)
     
434. RFLP - reading gels (reply: 1)
     
435. mutiple DNA bands on my agarose gel - (reply: 3)
     
436. RNA gel shift - (reply: 1)
     
437. smears on gel.. - REASONS (reply: 4)
     
438. gel purification of PCR product - (reply: 1)
     
439. which temperature should I run my 2. dimension gel at? - (reply: 3)
     
440. SDS-PAGE - CAN I USED 12% GEL FOR WHEAT LEAVE PROTEIN SDS-PAGE (reply: 1)
     
441. Etbr in agrose Gel - (reply: 5)
     
442. Metaphor agarose optimization - (reply: 1)
     
443. questions about native gel - (reply: 3)
     
444. Native Gel for QDs and Peptides - (reply: 8)
     
445. cannot see RNA on gel - (reply: 6)
     
446. Proteins samples not migrating from stacking into resolving gel - (reply: 6)
     
447. PAGE gel casting - (reply: 4)
     
448. Agarose gel for very small DNA fragments - (reply: 7)
     
449. Percentage of SDS- PAGE gel for co-IP - (reply: 1)
     
450. gel run - (reply: 1)
     
451. 2D Gel electrophoresis analysis software - Is a good one free available? (reply: 1)
     
452. fading DNA bands on agarose gel - is it normal? - (reply: 4)
     
453. SDS-PAGE over heating and will western work from that gel? - sds page running and western blot of gel run under too much heating (reply: 9)
     
454. PCR band disappears in agarose gel! - Agarose gel electrophoresis (reply: 4)
     
455. Minimum sample required for visualization on gel - How to load 1ul of DNA on gel (reply: 3)
     
456. MOLECULAR TEST WITH !JUST PCR AND GEL! FOR A STARTER - PLEASE GIVE ME SUGGESTIONS (reply: 3)
     
457. A native PAGE - for a protein, 15.3 kDa, gel/run conditions? (reply: 2)
     
458. Elution from immunoprecipitation suitable for 2D-GE - Elution from immunoprecipitation suitable for 2D gel electrophoresis (reply: 1)
     
459. sds page - how to quantifing of gel doc (reply: 1)
     
460. DNA concentration too low for gel electrophorsis - (reply: 6)
     
461. Acrylamide gels: sample preparation with high salt concentration - (reply: 1)
     
462. How to store PAGE gel and keep the bands after gel is run? - (reply: 22)
     
463. Electrophoresis: only seperate DNA by size!? - (reply: 4)
     
464. Gel ELECTROPHORESIS ?PLEASE HELP? - DNA ISOLATION (reply: 1)
     
465. Should I clone my PCR product even though it is invisible on gel? - (reply: 5)
     
466. staining the gel - (reply: 5)
     
467. How to do gel filtration - (reply: 3)
     
468. Probe labelling foe gel shifts - (reply: 2)
     
469. Small peptides in gel - What to do to see a 2kDa peptide in coomassie gel (reply: 25)
     
470. Western blot gel running problem - Gel Electrophoresis (reply: 7)
     
471. UREA in SDS-PAGE - How to make the gel (reply: 1)
     
472. 6% gel westren blot not working as well as 10% - (reply: 1)
     
473. TBE vs TAE for agarose gel electrophoresis - (reply: 32)
     
474. non-reductive gel to check the disulfide bond - (reply: 14)
     
475. Gel purified samples not sinking in gel well's - Not getting my samples to fall to bottom of well (reply: 8)
     
476. Why do we have to use MOPS and formaldehyde in RNA electrophoresis - (reply: 1)
     
477. Expired gel for WB - Can i still use it..(urgent) (reply: 4)
     
478. vertical agarose gel - (reply: 2)
     
479. A new plasmid appears on my gel in addition to my construct when I increase the - (reply: 4)
     
480. DNA purity by Agarose Gel Electrophoresis - (reply: 1)
     
481. plasmid DNA running on agarose gel to the opposite direction - (reply: 7)
     
482. problem with dna fragment extraction gel - (reply: 1)
     
483. biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (reply: 10)
     
484. Blue Native Gel Electrophoresis for membran protein complex - (reply: 1)
     
485. How do you store your gels? - (reply: 8)
     
486. Agarose gel for 580bp DNA - What are the suitable conditions? (reply: 8)
     
487. help with ligation and gel extraction - (reply: 5)
     
488. Problems with gel extraction and ligation - (reply: 12)
     
489. Why SDS page gel have to prepared freshly? - (reply: 6)
     
490. No PCR product detected in agraose gel - (reply: 7)
     
491. problem in my native gel - (reply: 4)
     
492. Does gel thickness affect light intensity of the DNA? - (reply: 3)
     
493. Native RNA gel - (reply: 1)
     
494. questions about the agarose bead protocol - (reply: 1)
     
495. Why can I not see bands on an agarose gel? - agarose gel visualization (reply: 5)
     
496. One specific DNA band became 2 bands after gel extraction? - (reply: 6)
     
497. using agarose for plates - (reply: 2)
     
498. no migration of DNA on electrophoresis - (reply: 7)
     
499. Problems in DNA sequencing after gel purification - (reply: 9)
     
500. Proteins and markers in top of gel - Running a gel (reply: 5)
     
501. Unwanted band seen along with my pcr product. - regarding PCR product with unwanted band formation during electrophoresis (reply: 3)
     
502. what is the meaning of de -gas the acrylamide gel mix? - (reply: 6)
     
503. SDS-PAGE gel preparation - (reply: 10)
     
504. sodium borate acid for DNA electrophoresis? - It's supposed to be much faster. Have you tried it? (reply: 13)
     
505. reuse PCR gel - (reply: 2)
     
506. which voltage for which agarose concentration? - assuming same size of gel, how should voltage be adapted to agarose concentratio (reply: 3)
     
507. Can we reuse agarose gel? - (reply: 10)
     
508. Question on Ethidium Bromide Gels - (reply: 2)
     
509. Allowed gel percentage to seperate proteins of 20kd or less - (reply: 1)
     
510. Gel percentage & cross-linker percentage, protein MW constrains. - (reply: 2)
     
511. Tris Tricine Gels for amyloid-beta Oligomers - (reply: 7)
     
512. extraction of DNA from agarose gels - (reply: 4)
     
513. Rhinovirus (RV) GEL FILTRATION - (reply: 1)
     
514. Storing gels . . . - (reply: 3)
     
515. protein diffusion in sds page gel - (reply: 2)
     
516. Streaks on 2D gels - (reply: 3)
     
517. to extract DNA from agarose gel - (reply: 11)
     
518. agar and agarose s - (reply: 4)
     
519. DNA agarose gels - trouble shoot weird gels (reply: 7)
     
520. eluting protein from the gel band... - (reply: 7)
     
521. Electrophoresis of Plasmid - (reply: 3)
     
522. Exposing southern gel to UV before transfer - (reply: 1)
     
523. Real Time: One Melting Curve 2 gel bands - (reply: 6)
     
524. Help needed-Protein extraction from native gel - (reply: 3)
     
525. gel shift assay help- big smear lane - (reply: 2)
     
526. Agarose gel electrophoresis - for separating bacteria cells?! (reply: 5)
     
527. formaldehyde in RNA gels - (reply: 4)
     
528. RNA gel troubleshooting - (reply: 8)
     
529. Strong distortion in bands of dna gel electrophoresis - (reply: 6)
     
530. Removal of etoh from purified samples - my gel purified samples contaminated with ethanol. how to get rid?? (reply: 6)
     
531. Unclear bands in bp marker of agarose gel - should i lower the voltage? (reply: 5)
     
532. Gel extraction - just wondering!!!! (reply: 8)
     
533. SDS-PAGE gel won't get solidified - (reply: 5)
     
534. Question about making low percent SDS-PAGE gels for big protein - (reply: 9)
     
535. Tricine SDS-PAGE gel under non-reducing - please tell me a protocol (reply: 1)
     
536. PFGE techinque - Pulse fiels gel electrphoresis (reply: 6)
     
537. Gel permeation & filtration - difference plz? (reply: 1)
     
538. Distinguish uncut vs. cut plasmids on agarose gel - (reply: 9)
     
539. FLAG M2 affinity gel agarose storage condition - (reply: 1)
     
540. protein contamination in DNA electrophoresis - (reply: 2)
     
541. protein elution from PAA gels - (reply: 2)
     
542. Problem in protein preparation in 2-D electrophoresis - (reply: 1)
     
543. Ramdom competitor in Gel Shift Assay - EMSA expert, please help! (reply: 1)
     
544. 2-D electrophoresis problem... strange spots - (reply: 2)
     
545. Denaturing Agarose gel for RNA - (reply: 10)
     
546. SDS-PAGE % - what % gel should I use (reply: 5)
     
547. Problem pouring SDS-PAGE gel - (reply: 9)
     
548. Multiple bands on RNA gel, why? - (reply: 6)
     
549. VERTICAL DGGE GEL - (reply: 5)
     
550. need help with calcium phosphate gel - (reply: 5)
     
551. Different conformation of DNA after gel extraction? - (reply: 6)
     
552. protein tranfer from gel to membrane, reversible ? - (reply: 2)
     
553. Agarose gels and SB Buffer - (reply: 7)
     
554. different amount of template DNA added will give out different pattern in gel af - (reply: 4)
     
555. electrophoresis to resolve two DNA of similar size - (reply: 6)
     
556. Should I use LMT Agarose to purify genomic DNA? Help please! - (reply: 1)
     
557. Help me! No colonies! Problem with gel purification? - (reply: 5)
     
558. Protease: Post Electrophoretic Reactivation - (reply: 3)
     
559. loading protein onto SDS-PAGE gel - (reply: 2)
     
560. PCR doesn't work! - Falling in an empty gel "space" (reply: 2)
     
561. which gel type to choose for protein electrophoresis? - difference between types of gels (reply: 12)
     
562. Gel filtration - (reply: 3)
     
563. Developing Gel instead of PVDF - (reply: 3)
     
564. Qiagen Gel Extraction of PCR product - (reply: 7)
     
565. gels for RNA - (reply: 1)
     
566. Reagents used...in restriction digestion and electrophoresis - (reply: 2)
     
567. RFLP electrophoresis - (reply: 2)
     
568. native gel -- protein migration - (reply: 1)
     
569. Problem choosing the right kind of Precast gels - (reply: 1)
     
570. how much do you load on the gel? - do you really dilute to certain OD? (reply: 3)
     
571. denaturing gel - (reply: 4)
     
572. curved gel surface - (reply: 10)
     
573. lousy agarose gel - (reply: 2)
     
574. problem with RNA ladder in polyacrylamide gel - (reply: 1)
     
575. gel purification necessary for ligation? - (reply: 3)
     
576. purification of DNA extracted from agarose gel - does ethidium bromide remains / interefrence with ligation (reply: 1)
     
577. two bands after gel elusion instead of one - (reply: 6)
     
578. problem with gel filtration - (reply: 1)
     
579. multiplex analysis of amplicons on gel using fluorophores - what fluorophore/dye can I use (reply: 3)
     
580. Gel Extraction problems - using Invitrogen Purelink (reply: 1)
     
581. protein precipitation during gel loading - (reply: 3)
     
582. Wierd 1xTAE Gel - (reply: 3)
     
583. Gel shift assay for non purified protein preps - (reply: 1)
     
584. Having problem preparing sample for 2D gel. Help pls. - (reply: 2)
     
585. SDS gel strangely running: curved ladder - Ladder starts running vertical, and then levels horizontal in the running gel (reply: 7)
     
586. Agarose gel - DEPC-induced fragility? - RNA gels (reply: 2)
     
587. Please help with high-molecular-weight DNA gel electrophoresis! - (reply: 2)
     
588. can you ligate gel slices directly? - (reply: 8)
     
589. how to estimate amount of DNA using gel? - do u use agarose or acrylamide? (reply: 2)
     
590. DNA fragmentation- gel electrophoresis assay - molecular biology (reply: 1)
     
591. Low melt temp agarose not melting - (reply: 13)
     
592. Gel Filtration - which system is most suitable? - (reply: 3)
     
593. denaturating protein electrophoresis - (reply: 5)
     
594. DNA migrates faster the longer you run a gel? - (reply: 4)
     
595. gel purification of the vector... - is it good enough for ligation? (reply: 7)
     
596. decontamination of electrophoresis chambers... - what solution should be used? (reply: 2)
     
597. Gel loading buffer - (reply: 12)
     
598. Gel elution of a band - (reply: 4)
     
599. Crystal Clear Acrylamide Gels - Has anyone been able to cast an optically clear Acrylamide gel? (reply: 4)
     
600. Gel Filtration/Protein Precipitation - (reply: 1)
     
601. FISH probes - why check with gel (reply: 1)
     
602. DGGE gel problem - (reply: 1)
     
603. Drying SDS gels - (reply: 4)
     
604. Efficiency of the transfer in Western Blotting - How can you tell from the gel? (reply: 10)
     
605. transverse agarose pore gradient gel electrophoresis - anyobdy gotta protocol?? (reply: 2)
     
606. Qiagen Rneasy multiple bands in gel - (reply: 1)
     
607. Band diffusion of my PCR product in electrophoresis - (reply: 3)
     
608. extra band after gel extraction - (reply: 4)
     
609. Does BSA slow DNA migration in agarose gels? - (reply: 1)
     
610. Help with western blotting - protein not running in to seperating gel (reply: 1)
     
611. Verticals streaks in kinase assay gels - (reply: 2)
     
612. how do you determine the correct voltage and time of running of an agarose gel? - (reply: 6)
     
613. Tips on Casting acrylamide gel - (reply: 4)
     
614. Urea gel - preparation of urea gel (reply: 2)
     
615. Plused Field Gel Electrophoresis - (reply: 1)
     
616. tricin gel problem - shrinking of stacking gel (reply: 6)
     
617. Storing SDS-page gels - (reply: 1)
     
618. Extract hemoglobin from blood for starch gel electrophoresis - (reply: 1)
     
619. Differenciation between plasmid, super-coiled DNA and linear DNA - What is the difference between plasmid and other DNA on the gel? (reply: 1)
     
620. Best electrophoresis set up? Biorad vs. Hoeffer - (reply: 4)
     
621. Acrylamide gel to resolve DNA of similar size? - a little advise please (reply: 8)
     
622. Strange analytical gel for miniprep - (reply: 4)
     
623. Immunoblot (western) resolution vs electrophoresis resolution - (reply: 1)
     
624. Softwares and hardwares used for scanning gel electrophoresis banding pattern - (reply: 1)
     
625. electrophoresis of insulin - (reply: 2)
     
626. DNA purification from agarose gels - (reply: 2)
     
627. DNA Purification from gels - (reply: 4)
     
628. Hand-held UV wand: the most useful thing ever? - Gel electrophoresis, southerns and northerns (reply: 7)
     
629. How to analyze antigen-antobody complex on gel? - Which gel will NOT break the complex? (reply: 3)
     
630. clarification - plasmid and gel - (reply: 9)
     
631. RT-PCR- help - Polyacrylmide Gel electrophrosis for PCR products (reply: 3)
     
632. No DNA on gel, but get PCR product - (reply: 2)
     
633. Weird Band gel migration - Molecular biology (reply: 7)
     
634. RNA sample stuck in well during agarose electrophoresis - (reply: 1)
     
635. NATIVE gel band - sample running at the side... - My NATIVE gel run produced a band which has hollowed out central with visible st (reply: 1)
     
636. SDS-PAGE running with my sample widening? - my sample sort of grew fat in the separating gel...why? (reply: 8)
     
637. how to interpret native gel result - (reply: 1)
     
638. question on complexes - protein-ligand complex detection in native gels (reply: 1)
     
639. gel purification after restriction digestion - (reply: 3)
     
640. Wrong band size using precast gel - (reply: 4)
     
641. No bands at all in gel for PCR products - (reply: 5)
     
642. Broken gel apparatus--easy fix? - (reply: 9)
     
643. AFLP gel staining trouble - (reply: 1)
     
644. tris-tricine gel transfer - (reply: 3)
     
645. RNA extraction with smear after electrophoresis - (reply: 1)
     
646. ssDNA smear during gel shift - (reply: 3)
     
647. pcDNA3.1 & pBIG2i vectors appear "bigger" on the gel! :( - (reply: 1)
     
648. ChIP sonication Input Agarose gel - (reply: 1)
     
649. Gel retardation - (reply: 1)
     
650. Two bands on a denaturing gel - always two bands on a denaturing gel with double stranded substrate? (reply: 1)
     
651. Checking integrity of total RNA using gel? - (reply: 6)
     
652. Introduced to Gel Retardation Assay - Today in practical class, our lecturer instructed us in GRA (reply: 2)
     
653. separation of pcr products of similar sizes in agarose gel - (reply: 1)
     
654. RNA Gel Problems - RNA not showing up as bands in gel (reply: 6)
     
655. resolution of similar size products using agarose gel - heeeeeelp !!! =P (reply: 10)
     
656. Agarose gel concentration for genomic DNA - (reply: 5)
     
657. SDS-PAGE gel drying - (reply: 8)
     
658. purification by gel? - after a first round of PCR (reply: 2)
     
659. human RNA contaminated with bacterial RNA - give your opinion about a RNA gel (reply: 8)
     
660. how to melt agarose, and keep it melted? - (reply: 3)
     
661. Help Needed! DNA sample dispersed b4 electrophoresis - (reply: 2)
     
662. gel and gelatin - (reply: 1)
     
663. Gel shift assay follow by Western blot analysis - Has anyone try this before? (reply: 8)
     
664. Gel Electrophoresis DNA base pairs size - (reply: 1)
     
665. U-shaped smear on EMSA gel - (reply: 1)
     
666. How to stain /destain quickly SDS-PAGE gel? - your patents? (reply: 13)
     
667. SOS: Gel purification of small size DNA - (reply: 6)
     
668. agarose gel storage - (reply: 6)
     
669. Gel Shift assays - (reply: 3)
     
670. RNA gel problems - (reply: 2)
     
671. protein did not enter the gel on EMSA - (reply: 2)
     
672. Question about gel purification of PCR product - (reply: 1)
     
673. IP and 2D gel analysis - buffer composition (reply: 1)
     
674. 2D electrophoresis - (reply: 1)
     
675. gel extraction problem - (reply: 6)
     
676. RNA Formaldehyde Gel problem - (reply: 5)
     
677. DNA Gel Extraction Problem - multiple bandings in gel extraction product (reply: 5)
     
678. Best Brand of Agarose? - (reply: 4)
     
679. Help with electrophoresis - Phosphate ester electrophoresis (reply: 1)
     
680. Smears on agarose gel, contamination? - (reply: 4)
     
681. Glycine in gel; fiber typing - (reply: 1)
     
682. Obtain larger dna fragments after digestion and gel cleaning - (reply: 2)
     
683. Genomic DNA on agarose gel - two bands of genomic DNA on agarose gel (reply: 2)
     
684. QIAquick Gel Extration Kit - (reply: 8)
     
685. Agarose / salmon sperm slurry - from upstate (reply: 5)
     
686. gel extraction of pcr products - (reply: 3)
     
687. do agarose gels expire? - (reply: 4)
     
688. Image processing of gel - (reply: 2)
     
689. non-specific bands in agarose gel - too many bands visible! (reply: 3)
     
690. Salmon sperm / agarose slurry - (reply: 3)
     
691. which agarose to use for pre-clearing - IP (reply: 1)
     
692. DNA concentration calculation from agarose gel - DNA concentration calculation (reply: 4)
     
693. shift of protein bands in SDS gel electrophoresis - where does it come from, can it be improved (reply: 2)
     
694. staining gels and western blot - (reply: 1)
     
695. agarose gel electrophoresis - (reply: 4)
     
696. best gel concentration and voltage - (reply: 3)
     
697. Is it possible to gel purify the ligated product? - Avoid non cut vector or self-liagted vector contam (reply: 2)
     
698. how to apply molecular weight marker on 2D gels? - application marker for 2D (reply: 1)
     
699. Odd curve of Gel purified PCR product - (reply: 5)
     
700. gel purification - (reply: 1)
     
701. Polyacrilamide Gel - (reply: 2)
     
702. agrose Vs. PAGE - (reply: 3)
     
703. RNA electrophoresis problem - RNA not denatured (reply: 9)
     
704. gDNA on a gel? - (reply: 7)
     
705. Gel Extraction of DNA - (reply: 5)
     
706. EMSA/Gel Shift wells problem - Protein stuck in the wells of EMSA gel (reply: 10)
     
707. Problem when making PAGE gel - (reply: 7)
     
708. can i autoclave agarose gel with TAE buffer - (reply: 4)
     
709. Dry SDS-PAGE gels - methods, tips to safely dry protein gels (reply: 2)
     
710. problem with agarose gel? - (reply: 11)
     
711. Polyacrylamide gel not polymerize - (reply: 5)
     
712. Protein not penetrating the resolving part of the gel - (reply: 2)
     
713. RNA isolation - i want to show a gel (reply: 4)
     
714. Rhinohide polyacrylamide gel - (reply: 6)
     
715. Identification of the cut and uncut plasmid on gel - (reply: 4)
     
716. Gel extraxction question - (reply: 6)
     
717. Power setting for electrophoresis - (reply: 5)
     
718. 2D electrophoresis of Plasma - (reply: 3)
     
719. Native Gel Staining?? - So I can electrolute proteins by cutting gel slice (reply: 4)
     
720. How to determine appropriate agarose % - (reply: 8)
     
721. Gel extraction and elution buffer - (reply: 4)
     
722. SDS-PAGE gel not polymerize - (reply: 11)
     
723. How to get sharp band on agarose gel? - (reply: 7)
     
724. Gel extraction problem - (reply: 15)
     
725. Sepharose or agarose in ChIP assay - (reply: 2)
     
726. Single cell gel electrophoresis (comet assay) - (reply: 1)
     
727. SDS-PAGE gel sticking - Gel sticking to the membrane (reply: 3)
     
728. problem with casting PAG - large number of persistent air bubbles in a