question on complexes - protein-ligand complex detection in native gels (Nov/11/2005 )
I am trying to detect complexes of fluorescent compound with my protein in the native gels but it seems like that the overall charge of the complex changes so much that it does not enter the gel at all (pH of the gel buffer - 8.8, run in the standard Tris-glycin buffer). Shall I change the pH of the gel buffer with HCl? Shall I also change the pH of the running buffer?
Thanks for the hints.
why don't you use exclusion chromatography to avoid any effect due to charges?...