Expected bands in agarose gel electrophoresis - (Oct/08/2006 )
I am running agarose 0.8% gel electrophoresis. I am using lambda DNA cut with Eco RI, Hind III, Bam HI. I will also cut pET-15b with Eco RI. I am using enzymes from promega. I need to find the expected bands of each. Where would I find this information?
find and download the sequence of lambda and pET-15b. Put said sequence into a programe like (vector NTI) then use the toolbar (analysis on VNTI) to cut the DNA with the appropirate enzyme. On VNTI there is a also a tool to display fragment sizes. One place to look is the company website (demo)
Thank you for your advice.