2D electrophoresis of Plasma - (Jun/16/2005 )
2D gel on Plasma look like crap. Same way to run on other tissue look good. Anyone have any suggestion?
-faipoon-
Have you removed your albumin first by a column?
Have you run an small 1D to check how your sample runs in an SDS-page?
Do you use high quality reagents?
Do you filter all your buffers previous to use?
If you are more precise at the pattern you get,maybe I can help better.
M
-marcfe-
QUOTE (marcfe @ Jun 19 2005, 02:23 AM)
Have you removed your albumin first by a column?
Have you run an small 1D to check how your sample runs in an SDS-page?
Do you use high quality reagents?
Do you filter all your buffers previous to use?
If you are more precise at the pattern you get,maybe I can help better.
M
Have you run an small 1D to check how your sample runs in an SDS-page?
Do you use high quality reagents?
Do you filter all your buffers previous to use?
If you are more precise at the pattern you get,maybe I can help better.
M
-faipoon-
Marc,
The problem is horizontal smearing? anyone have experience? see picutre? it suppose to glycoprotein> how can I make it look good?
-faipoon-