gel migration - DNA gel migration problem (Feb/28/2005 )

I trie to run my doubledigested plasmon (~4900bp) on a 1.5 % agarose gel, but it wount migrate from the well...why is this? the standrard and my pcr fragment (300bp) migrate just fine...

Hi

Your product should run the gel. I suggest using a lower concentration gel such as 1% or 0.75%, that will ensure that the DNA will pass through the pores of the gel. Another suggestion is to use freshly made gels and buffer, if these get too old then the capacity of the gel to transport the DNA is reduced.

Bob