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using agarose for plates - (Jul/06/2006 )

hi all!
i did one blunder... i added agarose instead of agar in lb for preparing plates for bacterial transformants... will it anyway affect my competent efficiency?


The bacteria will grow anyway. It will not affect either the growth or the transformation efficiency. The transformation efficiency doesn't have anything to do with the plate.


As dnafactory said, it'll grow.
Agar and agarose are actually almost the same thing, so no worry that they'll have different effect on bacterial or cell growth.

But it's certainly not a good thing to repeat often (haha) because the cost is almost 10 X different. Agarose more expensive than agar.


-I love MSGs!-