Gel extraxction question - (Jun/27/2005 )
I made lots of gel extraction using QIAquik kit, and i never had problems.
last time i found something strange in my extract... it seems gel!!!
how is it possibile?
I have used these same kits and found them to be quite reliable. Perhaps you got a bum column with a crack or hole in the frit allowing the agarose to flow thru?
i followed the protocol, but this time something didn't work....
i had 2 tubes, and in both i found gel
(2 different spin columns, sterile conditions etc etc)
By gel, do you mean actual gel, or white powdery stuff that looks like agarose?
I mean white powdery stuff that looks like agarose...
I am sticking to my story of column material that is flowing thru an imperfection in the column frit.
Spin the eluate 5 min 14k xg and take the supernate. Discard the pellet of white stuff. Check your plasmid on a gel.. if all looks good then go.
imperfection? in 2 different colums?
I worked out repeating the extraction following the protocol again.