Best Brand of Agarose? - (Sep/12/2005 )
What type of agarose does everyone think is the best? We are having troubles with ligation efficiency in the lab and my PI thinks that it might be some carry-over inhibitor form the agarose after we gel isolate with Qiagen Spin Columns?
It is easier to find a better purification kit rather than a better agarose gel.
I agree, it is probably something else affecting the ligation reaction; eg ATP gone bad. If your DNA is purified, it should be clean no matter where you get your agarose from.
I suspected from long time that qiagen gel extraction kit is not adviced for the purif for further ligation, so I ommited such step as far as possible. Make just a cut, purify it from the enzymes, and ligate all that you can together, later, in the screening, you will choose, what you need.
It's all about the purification kit.
Our lab at the Salk Institute uses the QIAquick gel extraction kit and EMD omnipure agarose with good results.