Agrose gel - (Oct/04/2008 )
I run my DNA sample in a agrose gel and then stain by ethidium bromide.
I often see bright line in the loading well after running gel for 2 hours. even after treating the sample by DNAse. Who can tell me what can cause the bright line in the loading well? Thank you
-AJIE-
QUOTE (AJIE @ Oct 5 2008, 05:51 AM)
I run my DNA sample in a agrose gel and then stain by ethidium bromide.
I often see bright line in the loading well after running gel for 2 hours. even after treating the sample by DNAse. Who can tell me what can cause the bright line in the loading well? Thank you
I often see bright line in the loading well after running gel for 2 hours. even after treating the sample by DNAse. Who can tell me what can cause the bright line in the loading well? Thank you
Something odd here. Do you mean RNA sample, treated with DNAse, or DNA sample treated with RNAse?
-perneseblue-
QUOTE (perneseblue @ Oct 4 2008, 11:14 PM)
QUOTE (AJIE @ Oct 5 2008, 05:51 AM)
I run my DNA sample in a agrose gel and then stain by ethidium bromide.
I often see bright line in the loading well after running gel for 2 hours. even after treating the sample by DNAse. Who can tell me what can cause the bright line in the loading well? Thank you
I often see bright line in the loading well after running gel for 2 hours. even after treating the sample by DNAse. Who can tell me what can cause the bright line in the loading well? Thank you
Something odd here. Do you mean RNA sample, treated with DNAse, or DNA sample treated with RNAse?
DNA SAMPLE treated by DNAse. I want to chop the bid DNA molecular
-AJIE-
could u kindly provide us with a gel picture by any chance?
-Hanming86-
hmmm... that rules out genomic DNA. Well I would guess that the DNA sample is contaminated. Proteins/polysaccarides contaminating the preparation would appear a stuck near the well. Phenol-chloroform extraction would recover this form of contamination.
-perneseblue-