Protocol Online logo
Top : Forum Archives: : Protein and Proteomics

extra bands on SDS PAGE gel - (Oct/31/2007 )

hi all! i have successful expressed my protein for about a yr now. recently however i have upscaled the reaction (only to 1L!) and have had to change my buffer for concentrating in as the protein precipiated out. the composition of this buffer is as follows: Tris Hcl 50mM
NaCl 150mM
Mgcl2 1mM
Now i am getting two bands on my SDS PAGE gel (normally had only 1 at 25kDA) at 25kDA and 50kDa. homologs of this protein has been known to form a dimer. any ideas if this is what is happening here or is there something ive missed that has gone wrong??


I think as per your information it might be dimer.


I am guessing its the dimer too


I make a bet you have dimer formation


QUOTE (Missele @ Nov 1 2007, 11:42 AM)
I make a bet you have dimer formation

thanks all that puts me at ease!