Improved electrophoresis of nucleic acids. - (Aug/09/2008 )

Hello,
I think some of you may find this interesting and/or useful.
Science Daily articles:
http://www.sciencedaily.com/releases/2004/...40210075111.htm
http://www.sciencedaily.com/releases/2007/...70718162904.htm
Relevant papers:
http://www.ncbi.nlm.nih.gov/pubmed/14989083
http://www.ncbi.nlm.nih.gov/pubmed/15517972
And patent:
http://v3.espacenet.com/textdoc?DB=EPODOC&...7131553&F=0
(To download patent click "Original document" and then "Save Full Document".)

I think it's indeed very interesting!! Do you have PDF files of the articles?

Best regards,
Jackson

No, I don't. The patent seems to have quite a lot of information though. (I haven't read it because I'm not working with nucleic acids.)

OK I will check that out!
Thanx!

I can send you the article, if you want.

If you could send it to me also, my friends would appreciate it.

There's been a fair amount of discussion here on these SB buffers -- see here (from July 2005), here (from September 2005), and here (from October 2005).

The borate system works very well for short, fast gel runs, but I wouldn't recommend it if you have a large gel format and you want to run for a long time. I usually do 250-300V for 10 minutes on a 0.8% gel.

Having said that, the resolution can be great. It's relatively easy to separate 500 from 517 bp in a ladder on a 0.8% gel.

Does anyone know if using the SB buffer would affect a subcloning procedure? We use TAE now and it works fine, but it would be great to run it faster if at all possible. I just don't want to cut the gel out and have the SB mess up the finicky nature of DNA Ligase or disturb the balance of the ligation buffer.


-Jerry