Gel Shifts: Non-Specific Competition - (Oct/26/2006 )
I am driving myself crazy trying work out molar excess of poly d[I] poly d[C] for use in gel shift assays. I am using it as a non-specific competitor. How can I work out the molarity of a 1ug/ul solution whne the size ranges from 300-20kb?
Any would be much appreciated!
For poorly defined polymers, it is almost always best to think in terms of the molarity of the monomer rather than the polymer. The monomer unit in this case would be either the dI or dC, or the dI-dC pair.