phenol/CHCl3 vs purification from gel - (Oct/02/2006 )
I want to dephosphorylate my vector with the CIP phosphatase (NEB) which can not be heat inactivated. How do I lose less DNA when trying to get rid of the phosphatase? By purification from gel or by phenol/chloroform precipitation? Any suggestions appreciated!
i was gel purifying my vector after CIP treatment. i was using QIAGEN QIAquick gel extraction kit. but nowadays i am using PCR purification kit to purify my vector after CIP treatment. (this is the easiest way of doing it-but check that kit is suitable for purification after CIP-QIAGEN's PCR purification kit is ok for this) yield is high with pcr purif. kit.