Viral RNA - have you ever run it in a gel? (Nov/11/2006 )
Dear all,
I am just wondering what should I expect to see when I run HIV viral RNA in a gel... should be on acrylamide or agarose?
I think there should not be visible "subunits" but a band reflecting the viral genome?
What do you think guys?
I was using acrylamide gel. Howeve, I did not see any band.
My samples are mainly from treated petient serum, so viral load is low.
You should be see a band, but to confirm this you have to hybridized with you specific probe.
Best regards
My samples are mainly from treated petient serum, so viral load is low.
You should be see a band, but to confirm this you have to hybridized with you specific probe.
Best regards
well acrylamide is used for protein and DNA fragments not greater than 4 kb...did you try it with agarose gel?
yes I did, since I was expecting to see a band for the viral genome which is about 10Kb
I was using acrylamide gel. Howeve, I did not see any band.
My samples are mainly from treated petient serum, so viral load is low.
You should be see a band, but to confirm this you have to hybridized with you specific probe.
Best regards
well acrylamide is used for protein and DNA fragments not greater than 4 kb...did you try it with agarose gel?
so ur saying it also did not go well with agarose gel?
nop, despite the A260/280 were 2.0 and I loaded 250 ng...
The agarose gel will be a good choice, but you have to get a good viral DNA extraction.