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DGGE and Smiling bands! - Side bands on my DGGE gel keep smiling! (Jan/18/2005 )

Hi! we've just got some new DGGE apparatus for our lab and after several attempts at running gels of a 40-70% gradient we always seem to get really bad smiling of the side wells. We run it over night at about 66volts. Any ideas how we can stop the smiling? ph34r.gif

-Oral micro-

Concentration salt in buffer?

Keep on smiling biggrin.gif


Do you have the sides of the plate closed off with electrical tape while running the gel?