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Drying SDS gels - (Jan/12/2006 )

I need an alternative method for drying SDS gels because my drying machine is giving me the headache, it doesn't want to vacuum good or dry wll, it's still wet when I open it so, yeah, help!? LOL

-smoochiepie79-

hello

try this

http://www.bio-rad.com/B2B/BioRad/product/...vel=Lit+Request

it's air drying, takes 24 hours, however, you get a really nice result. especially if you want to scan your gels for publication

I only use the drying frames, but not the dryer itself (not bought).

I would recommend however to soak the gel an cellophane in a drying solution : 30% methanol and 3 % glycérol to avoid that the gel cracks.

-laurence-

QUOTE (laurence @ Jan 12 2006, 11:33 AM)
hello

try this

http://www.bio-rad.com/B2B/BioRad/product/...vel=Lit+Request

it's air drying, takes 24 hours, however, you get a really nice result. especially if you want to scan your gels for publication

I only use the drying frames, but not the dryer itself (not bought).

I would recommend however to soak the gel an cellophane in a drying solution : 30% methanol and 3 % glycérol to avoid that the gel cracks.


Thanks, but my PI is not willing to order that, it sucks.

-smoochiepie79-

We have no drying machine or frames so to dry our gels we put them on filter paper with a layer of cellophane over the top and squash them under somthing heavy (and old catalogue is good). However this takes about a week!

-Rosie-

ok,

other solution would be to stick it on a glass, put some cellophane and stick some adhesive ruban around, let it dry, takes several days, scan it.
however I find it difficult to dry completely, and after more than one week, I stuck the gel in my labbook and then I realized that it was catching the wet around, in atmosphere, and the page beside the gel was no more flat and so on.
however you can keep the scan.

-laurence-