Uncut genomic DNA - Migrate through the gel (Nov/26/2006 )
During agarose gel electrophoresis my uncut genomic DNA has migrate approx. third of the way down the gel. Why is this? It is meant to stay close to the well.
i am not sure i understood what you mean. it will appear as many bands, usually higher than the cut one.
where is your uncut DNA in relation to the DNA ladder?
may you post a picture of your gel? or be more precise in your exp description ?