Silica Gel Chromatography beginners questions - Need some help getting started... (Apr/09/2008 )
Hello guys, I need to separate UDP-glucose from glucose on a silica gel-column.
However, I'm pretty new to all this so I'm confused by the "mesh" measurment. Is this dependant on the silica gel bead size? Porosity? Both?
Any other general tips for performing this type of column chromatography?
Anything would be appreciated.
mesh is particle size. the higher the mesh number, the smaller the particle.
as for advice, tell us your protocol and we may be able to give some advice.
just two pieces of advice now, try to maintain a constant flow rate and not too fast or you will compress the bed and kill the flow.