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Primer Related Discussions
  1. need help...how to compare mouse vs human sequences for primer design - (reply: 2)
  2. Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
  3. Primer design for PCR following ChIP - (reply: 3)
  4. Primer dimers!? HELP - (reply: 8)
  5. How primers can be measured? - (reply: 6)
  6. What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
  7. how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
  8. concentration of primers - (reply: 1)
  9. Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
  10. Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
  11. cDNA and primer Design Help - (reply: 6)
  12. Primer design program for Methylight - (reply: 3)
  13. Please help me design MSP primer! - (reply: 4)
  14. Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
  15. RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
  16. cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
  17. Designing Primers - (reply: 1)
  18. Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
  19. qRT-PCR primer design - (reply: 1)
  20. PCR primers + large mRNA amplification - (reply: 5)
  21. help design primer - (reply: 6)
  22. Different efficiency in different samples but the same primer - (reply: 1)
  23. primers amplify additional band (!) - (reply: 1)
  24. Designing the primers for qRT PCR - Query - (reply: 14)
  25. primer designing - (reply: 1)
  26. Primer Design for nucleotide sequence - (reply: 1)
  27. Attaching DNA bases - Creating primers and plasmids? (reply: 3)
  28. Why the addition of restriction sites to primers? - (reply: 3)
  29. Primer analysis sites - Any suggestions? (reply: 3)
  30. please help me to be sure from design primer have it - (reply: 3)
  31. manual primer desgining - (reply: 2)
  32. newbie question on Primer design for MSP - (reply: 2)
  33. Confirming published methylation primers - (reply: 1)
  34. Primer design - How to - am a novice ? (reply: 6)
  35. SNP - How to design the SNP primer (reply: 1)
  36. how to synthesize the pcr primers - (reply: 3)
  37. incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
  38. RT-PCR primer design and in silico PCR - (reply: 4)
  39. primer design - over expression (reply: 1)
  40. Primer design and tolerances - (reply: 3)
  41. help with degenerate primer design - (reply: 1)
  42. mutations in primer sequence - (reply: 2)
  43. Primer design - Primer design for PCR with TE sites (reply: 2)
  44. primer conditions for expression work - (reply: 5)
  45. promoter pcr using primers with restriction sites giving trouble - (reply: 7)
  46. primer Question pVax1 RE / Kozak - (reply: 1)
  47. Primer design for SNP detection - (reply: 2)
  48. max primer length - 85 nt too long? - (reply: 5)
  49. recommended primers for caspase 8 & 9 - human (reply: 4)
  50. Optimising bisulphite PCR primers - (reply: 4)
  51. Determine specific primers and transgene copy number by PCR - (reply: 1)
  52. Any Universal Primer for 3' of cDNAs of all mRNAs? - (reply: 2)
  53. can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
  54. conc of cDNA to be used - PCR to test primers (reply: 5)
  55. please help me check BSP primers. - (reply: 6)
  56. primer design - how far apart Tms could be? (reply: 4)
  57. a problem about the Tm of the BSP primer - (reply: 2)
  58. primer design from mRNA - (reply: 12)
  59. Please help me design BSP primer. - (reply: 14)
  60. Storing primers...together? - (reply: 3)
  61. different length for primers amplifying - (reply: 2)
  62. Primer concentration is quantitative Real-time PCR - (reply: 1)
  63. design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
  64. some primer dilution calculations - (reply: 7)
  65. TOPO primer choice for sequencing - (reply: 3)
  66. Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
  67. primer desighning with RE sites - (reply: 5)
  68. Primers for Real time PCR - Really need some advice (reply: 1)
  69. second strand cDNA synth without primer? - (reply: 2)
  70. General question about restriction sequences in PCR primers - (reply: 3)
  71. chip assay primers - chip assay primers (reply: 2)
  72. Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
  73. How to ressuspend primers for Sybr? - (reply: 3)
  74. picking primers - (reply: 4)
  75. primer/probe mix for standard generation? - (reply: 2)
  76. restriction enzyme site with primers - (reply: 4)
  77. weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
  78. Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
  79. figure out the size of amplicon using known primer - (reply: 3)
  80. microgram per microlitre primer to molarity conversion.. - calculation plss (reply: 1)
  81. ***MSP Primer Troubleshooting Specificity*** - (reply: 6)
  82. How good are my PCR primers? can I go with it?! - (reply: 2)
  83. primer design for unknown cDNA sequence - (reply: 1)
  84. primer Tm for PCR mutagenesis - (reply: 1)
  85. Primer design - Issues with GAPDH primer blast search (reply: 2)
  86. sequence retrieval for primer design - (reply: 2)
  87. Does anyone know how to design LUX primers - (reply: 2)
  88. PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
  89. Mutagenesis and primer purity? - (reply: 4)
  90. I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
  91. PCR primers with overhangs - (reply: 2)
  92. Designation of PCR primers in ChIP experiment - (reply: 3)
  93. Primer express? - Do you use it? (reply: 2)
  94. What is the proper upper limit of false priming in real time? - (reply: 3)
  95. gene-specific Primer for qRT-PCR - (reply: 2)
  96. primer design for PCR cloning - (reply: 1)
  97. PCR primers artifact - (reply: 2)
  98. Primer Express by ABI - (reply: 2)
  99. what affects primer specificity? - (reply: 2)
  100. Primers PCR - storage (reply: 5)
  101. Human Epigenome Project primers? - (reply: 2)
  102. Degenerate Primers - Help with Degenerate Primers (reply: 1)
  103. ready to use primers for BSP - (reply: 4)
  104. what's the difference between sequencing primer and cloning one. - confused (reply: 5)
  105. GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
  106. FGF20 PRIMER - (reply: 2)
  107. primer design for Syber Green - (reply: 1)
  108. primer walking design tool - (reply: 1)
  109. primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
  110. Degenerate Primers and dI - (reply: 5)
  111. designing a primer - (reply: 5)
  112. using primers programms - (reply: 9)
  113. PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
  114. long range PCR primer design - (reply: 1)
  115. what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
  116. How to get rid of primer dimers - (reply: 2)
  117. one nucleotide in the primer is wrong - (reply: 2)
  118. real time PCR using two sets of primers with different melt curves - (reply: 4)
  119. Introduce restriction sites with PCR primers - (reply: 2)
  120. MSP Primer design -rules to choose primers and reasons - (reply: 2)
  121. Primer calculation - (reply: 3)
  122. Testing newly designed primers prior to real time - (reply: 2)
  123. information regarding PCR using very long primers - (reply: 4)
  124. Bisulfite: PCR condition or primer problem? - (reply: 13)
  125. storage of primers - (reply: 3)
  126. too many hits on Blast for primers / microsatellite - (reply: 4)
  127. sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
  128. Primer orientation, forward and reverse - (reply: 4)
  129. Negative control primer for ChIP - (reply: 3)
  130. RT-PCR primer designing - (reply: 6)
  131. Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
  132. My primers don't work with Chip - (reply: 1)
  133. qPCR using random primers - (reply: 4)
  134. Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
  135. Amplifying a 1700bp fragment using a mismatched primer pair - (reply: 2)
  136. Degenerate Primers - (reply: 12)
  137. Plasmid DNA Sequencing with Universal Primers - (reply: 3)
  138. Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
  139. Possible mutations in primers? - (reply: 4)
  140. Need to confirm PCR primers are bad - (reply: 5)
  141. Help! Primer Dimers… - (reply: 3)
  142. Very low PCR primer concentrations? - (reply: 4)
  143. Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
  144. Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
  145. False priming in MSP - (reply: 4)
  146. Can primers go bad and give a smear in PCR? - (reply: 12)
  147. QuickChange primers self-made - (reply: 4)
  148. PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
  149. digoxygenin-labelled primer - (reply: 3)
  150. freezing of primers - (reply: 2)
  151. Primer dilution and calculation - (reply: 4)
  152. Designing primer for Banana mild mosaic virus - (reply: 3)
  153. primer for real-time and reverse transcriptase PCR - (reply: 1)
  154. Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
  155. humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
  156. 5' Primer modification - Tm-effect ?? (reply: 1)
  157. sequencing - which primer should I trust? (reply: 4)
  158. Help- degenerate primer design - (reply: 4)
  159. Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
  160. Is a Kazark sequence needed in a upper primer? - (reply: 17)
  161. cytokines primers - (reply: 1)
  162. Inverse PCR primers design - (reply: 4)
  163. general pcr/primer question - (reply: 1)
  164. primers in PCR - primer removed or not (reply: 5)
  165. different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
  166. Primers for BS treated DNA - (reply: 5)
  167. concentration of primers - (reply: 5)
  168. forward & reverse primers - (reply: 3)
  169. Primers & probes of Applied Biosystems for real time - (reply: 2)
  170. Methyl Primer Express from ABI - (reply: 2)
  171. design primer - (reply: 5)
  172. can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
  173. primer design questions for confused - (reply: 2)
  174. primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
  175. primer concentration - (reply: 7)
  176. RT-PCR primer design: Over introns and exons? - (reply: 2)
  177. SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
  178. pcr yield and primer concentration - (reply: 5)
  179. Primer design with restriction sites - (reply: 3)
  180. differences between oligo and random primers - (reply: 2)
  181. Any primer design programs...? - (reply: 8)
  182. Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
  183. primer : shifting peak - (reply: 4)
  184. Designing primers and hybridization probes - (reply: 1)
  185. ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
  186. Methylation specific primer designing - (reply: 1)
  187. Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
  188. RT-PCR primer design - (reply: 1)
  189. how to check primers - (reply: 4)
  190. TM for primers with restriction secuence tail. - Suggestion (reply: 3)
  191. How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
  192. PCR with 75 mer forward primer - (reply: 4)
  193. about the primers in MSP - (reply: 5)
  194. primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
  195. PCR, labelled vs unlabelled primers - (reply: 4)
  196. Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
  197. Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
  198. The weirdest primer ever - (reply: 7)
  199. no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
  200. What happens to PRIMERS at 72 C ? - (reply: 5)
  201. Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
  202. Direct BSP using tag-modified primers - (reply: 2)
  203. Mutagenesis using 4 sets of primer - (reply: 6)
  204. PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
  205. pro-PCR with only one primer... - to check directionality. (reply: 4)
  206. cDNA synthesis primer - (reply: 3)
  207. antigen primers - (reply: 1)
  208. Annealing vs melting Ts for real time PCR primers - (reply: 1)
  209. Calculating Tm for bisulfite primers? - (reply: 3)
  210. Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
  211. Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
  212. Primer for 5' RACE - (reply: 2)
  213. ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
  214. PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
  215. PCR primers with high Tm - (reply: 6)
  216. Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
  217. making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
  218. Bisulfite sequencing primer - (reply: 4)
  219. random primers for qPCR - (reply: 3)
  220. difficulty in Primer walking - (reply: 2)
  221. Primer for sequencing - need help!!!!!!!!!! (reply: 6)
  222. Primer design invert pcr - (reply: 5)
  223. Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
  224. Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
  225. BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
  226. dimers of dna (not primers) following digest or pcr? - (reply: 11)
  227. Question about Realtime PCR primer concentration.... - (reply: 5)
  228. plasmid amplification - exons and primers (reply: 1)
  229. reverse primer design with restriction site - (reply: 6)
  230. question in 5' RACE and primer extesion - (reply: 8)
  231. PCR product dimer (not primer dimer!) - PCR (reply: 2)
  232. from which strand to derive BSP primers? - (reply: 5)
  233. Primer Annealing for PCR - (reply: 4)
  234. Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
  235. How to design primer for sequencing unknown DNA - (reply: 5)
  236. need helps on probe design--human ip10 primers and probe. - (reply: 2)
  237. Sequencing Primers - (reply: 4)
  238. RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
  239. Primer design with incomplete sequence - (reply: 2)
  240. PCR primers from amino acids? - (reply: 1)
  241. primers design for mutagenesis of two nearby sites - mutations (reply: 2)
  242. Intensive primer dimers - (reply: 2)
  243. PCR is positive with one set of primers, negative with another. - (reply: 4)
  244. Trouble with primers of BSP - (reply: 3)
  245. potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
  246. primer design - how to design primers (reply: 2)
  247. Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
  248. Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers?&# - (reply: 6)
  249. southern hybridizaion with 5' end labelled primer - (reply: 1)
  250. Disadvantages of using oligo-dT primers - (reply: 1)
  251. do primers and dNTPs alter digestion efficiency? - (reply: 4)
  252. PCR with degenerate primer - (reply: 6)
  253. RT-PCR primers for E. coli LacZ gene - (reply: 3)
  254. primer reconstitution - (reply: 2)
  255. Primer for 4 genes sharing short consensus sequence - (reply: 2)
  256. Looking for online primer design for SNaPshot - (reply: 1)
  257. PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
  258. multiplex PCR for three sets of primers - (reply: 1)
  259. How many mismatches in point mutation primers? - (reply: 3)
  260. single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
  261. Mouse MSP primers - (reply: 11)
  262. Primer Dimer problems in real-time PCR - (reply: 14)
  263. Degenerate primers for nested PCR? - (reply: 1)
  264. Reliable ChIP primers for Sp1? - (reply: 2)
  265. increased primer dimer with template - (reply: 2)
  266. free software to examine if two primers will make dimers - (reply: 3)
  267. Testing primers - (reply: 3)
  268. Two round MSP with the same primer - (reply: 1)
  269. Designing a Primer within an exon - Consesus required - (reply: 2)
  270. difference between primer extension and RACE - (reply: 6)
  271. Primer - (reply: 1)
  272. rat 18s rRNA primer - designing primers (reply: 1)
  273. Designing primers for multiplex - BLASTing lots of primers (reply: 3)
  274. Primer design question - Use of primer 3 (reply: 3)
  275. RT-PCR Primer design - which region? - (reply: 2)
  276. No PCR products in MSP with MethPrimer designed primers - (reply: 2)
  277. design primer for PCR.. - (reply: 3)
  278. Design primer - (reply: 2)
  279. extra sequence in primers - (reply: 4)
  280. calculating Tm of primers - (reply: 8)
  281. How to design random primers - (reply: 2)
  282. PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
  283. primer design problem - (reply: 1)
  284. 60 bp PCR and primer dimers - (reply: 6)
  285. Primer Express Software - Probe Design (reply: 8)
  286. Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
  287. designing flanking primers around msp primers - (reply: 1)
  288. BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
  289. primer does not bind - help (reply: 2)
  290. SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
  291. RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
  292. is it effective to use a primer of SD rat to Wistar rat in PCR - help (reply: 2)
  293. Primers for RT reaction - (reply: 6)
  294. How to prepare primer solution - (reply: 7)
  295. primer storage - (reply: 7)
  296. Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
  297. reducing primer dimer - (reply: 1)
  298. primer melting temperature - (reply: 2)
  299. end of primer - (reply: 2)
  300. PCR primer dimer? - (reply: 7)
  301. PCR primer Tm and concentration question - (reply: 3)
  302. cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
  303. primer design - checking off-target site - (reply: 5)
  304. Which RT primer is better for qPCR - (reply: 2)
  305. Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
  306. primer optimization - (reply: 1)
  307. multiplex PCR - template and primer concentration (reply: 2)
  308. Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
  309. The perfect number of CpGs in MSP primer - (reply: 8)
  310. Primers - (reply: 1)
  311. Probes or primers that span introns - (reply: 6)
  312. what primers specific for B.stearothermophilus?Help pls. - (reply: 5)
  313. problem with my PCR - primer dimer. - (reply: 1)
  314. how long a primer should I use? - (reply: 1)
  315. what is the "tailed primer and taq primer"? - (reply: 2)
  316. Difficutly go separate product from primer dimers - Methylation specific PCR (reply: 1)
  317. designing gene-specific primer for cDNA synthesis - (reply: 5)
  318. How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
  319. software to find restriction site and TM for primer - (reply: 7)
  320. product size for real-time PCR primer - (reply: 3)
  321. real time PCR primers - (reply: 2)
  322. cpgware primer design, does it work - (reply: 4)
  323. PCR Primer Design with restriction sites - (reply: 1)
  324. Sequence input for bisulfite PCR primer design - (reply: 1)
  325. Database software to catalogue / archive primers - Database software to catalogue / archive primers (reply: 1)
  326. primer design for bisulphite treated DNA - (reply: 1)
  327. primer design: end-point vs. RT - (reply: 1)
  328. software to check the primer dimer - (reply: 3)
  329. BSP and MSP primer check - (reply: 2)
  330. How to get rid of PCR primer dimer - (reply: 65)
  331. RT-PCR primer design - exon-exon primer design (reply: 5)
  332. availabre primers for MSP - (reply: 1)
  333. RACE - specific primers in first strand synthesis (reply: 4)
  334. Primer Designer Online - Pipleline use of designer (reply: 1)
  335. methylation primers - problems with primers (reply: 1)
  336. real-time PCR - How to design primers (reply: 2)
  337. PCR primers - PCR primers for RT-PCR (reply: 1)
  338. Primers: advice? - (reply: 1)
  339. bisulfite pcr primers - (reply: 1)
  340. RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
  341. Double strand cDNA sysnthesis - Using random primers (reply: 1)
  342. Need suggestions for degenerate primers - (reply: 2)
  343. Primer design - Primer design for RT-PCR (reply: 1)
  344. Primer contamination - (reply: 2)
  345. questions about degenerate primer PCR - (reply: 2)
  346. How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
  347. primer purification - search method for primer purifying (reply: 3)
  348. Software for MSP (methylation-specific PCR) primer design - (reply: 3)