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Primer Related Discussions
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need help...how to compare mouse vs human sequences for primer design - (reply: 2)
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Create an artificial restriction site for a endonuclease and 53bp long primer? - (reply: 2)
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Primer design for PCR following ChIP - (reply: 3)
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Primer dimers!? HELP - (reply: 8)
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How primers can be measured? - (reply: 6)
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What are normalisation primers for the rt-PCR? - Im going to order the mirVana rt-PCR kit for miRNAs (reply: 1)
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how 2 design rev.transPCR primer? - need functioning protocol.... (reply: 1)
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concentration of primers - (reply: 1)
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Primer design from mRNA or DNA - Confused about what sequence to use for design of GSP and pPCR primers (reply: 2)
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Questions about primer design for BSP in plant - shouldn't we take the mC in CHG CHH context into consideration? (reply: 3)
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cDNA and primer Design Help - (reply: 6)
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Primer design program for Methylight - (reply: 3)
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Please help me design MSP primer! - (reply: 4)
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Tm of the primers - company sends primers with lower Tm than expected (reply: 3)
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RT-PCR primer design - RT-PCR primer design...need ur help (reply: 1)
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cDNA and additional bands when run on gel - I get a band o fthe same Mw regardless of the primers I use (reply: 2)
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Designing Primers - (reply: 1)
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Standard sequencing primers - how to find which sequencing primers bind to my DNA? (reply: 2)
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qRT-PCR primer design - (reply: 1)
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PCR primers + large mRNA amplification - (reply: 5)
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help design primer - (reply: 6)
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Different efficiency in different samples but the same primer - (reply: 1)
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primers amplify additional band (!) - (reply: 1)
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Designing the primers for qRT PCR - Query - (reply: 14)
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primer designing - (reply: 1)
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Primer Design for nucleotide sequence - (reply: 1)
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Attaching DNA bases - Creating primers and plasmids? (reply: 3)
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Why the addition of restriction sites to primers? - (reply: 3)
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Primer analysis sites - Any suggestions? (reply: 3)
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please help me to be sure from design primer have it - (reply: 3)
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manual primer desgining - (reply: 2)
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newbie question on Primer design for MSP - (reply: 2)
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Confirming published methylation primers - (reply: 1)
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Primer design - How to - am a novice ? (reply: 6)
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SNP - How to design the SNP primer (reply: 1)
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how to synthesize the pcr primers - (reply: 3)
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incorporation of restriction site in Primers - Help to design primer to clone gene in frame with fluorescent tag (reply: 2)
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RT-PCR primer design and in silico PCR - (reply: 4)
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primer design - over expression (reply: 1)
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Primer design and tolerances - (reply: 3)
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help with degenerate primer design - (reply: 1)
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mutations in primer sequence - (reply: 2)
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Primer design - Primer design for PCR with TE sites (reply: 2)
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primer conditions for expression work - (reply: 5)
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promoter pcr using primers with restriction sites giving trouble - (reply: 7)
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primer Question pVax1 RE / Kozak - (reply: 1)
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Primer design for SNP detection - (reply: 2)
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max primer length - 85 nt too long? - (reply: 5)
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recommended primers for caspase 8 & 9 - human (reply: 4)
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Optimising bisulphite PCR primers - (reply: 4)
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Determine specific primers and transgene copy number by PCR - (reply: 1)
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Any Universal Primer for 3' of cDNAs of all mRNAs? - (reply: 2)
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can you judge a 22bp primer based on the 3' 10bp? - (reply: 6)
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conc of cDNA to be used - PCR to test primers (reply: 5)
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please help me check BSP primers. - (reply: 6)
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primer design - how far apart Tms could be? (reply: 4)
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a problem about the Tm of the BSP primer - (reply: 2)
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primer design from mRNA - (reply: 12)
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Please help me design BSP primer. - (reply: 14)
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Storing primers...together? - (reply: 3)
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different length for primers amplifying - (reply: 2)
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Primer concentration is quantitative Real-time PCR - (reply: 1)
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design primers with custom end - To clone my gene into the MCS as fusions to the GFP tag (reply: 5)
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some primer dilution calculations - (reply: 7)
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TOPO primer choice for sequencing - (reply: 3)
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Anchored oligo dT and Thermostable reverse transcriptase - temperature compatibility between RT and oligo dT priming (reply: 8)
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primer desighning with RE sites - (reply: 5)
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Primers for Real time PCR - Really need some advice (reply: 1)
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second strand cDNA synth without primer? - (reply: 2)
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General question about restriction sequences in PCR primers - (reply: 3)
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chip assay primers - chip assay primers (reply: 2)
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Questions about Stratagene QickChange Multi-mutagenesis - about how many primers and template DNA size (reply: 5)
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How to ressuspend primers for Sybr? - (reply: 3)
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picking primers - (reply: 4)
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primer/probe mix for standard generation? - (reply: 2)
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restriction enzyme site with primers - (reply: 4)
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weird problem with Primers! - problem with the gene sequence i used to design my primers (reply: 4)
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Primer mismatch bother me! - when enzyme sites added at 5' ends, mismatch increases. (reply: 1)
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figure out the size of amplicon using known primer - (reply: 3)
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microgram per microlitre primer to molarity conversion.. - calculation plss (reply: 1)
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***MSP Primer Troubleshooting Specificity*** - (reply: 6)
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How good are my PCR primers? can I go with it?! - (reply: 2)
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primer design for unknown cDNA sequence - (reply: 1)
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primer Tm for PCR mutagenesis - (reply: 1)
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Primer design - Issues with GAPDH primer blast search (reply: 2)
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sequence retrieval for primer design - (reply: 2)
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Does anyone know how to design LUX primers - (reply: 2)
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PCR primers for CRISPR - primer3 isn't helpful in this case (reply: 1)
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Mutagenesis and primer purity? - (reply: 4)
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I have a problem with the M13 tailed primer method - M13 tailed primers-SSR (plants) (reply: 1)
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PCR primers with overhangs - (reply: 2)
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Designation of PCR primers in ChIP experiment - (reply: 3)
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Primer express? - Do you use it? (reply: 2)
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What is the proper upper limit of false priming in real time? - (reply: 3)
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gene-specific Primer for qRT-PCR - (reply: 2)
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primer design for PCR cloning - (reply: 1)
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PCR primers artifact - (reply: 2)
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Primer Express by ABI - (reply: 2)
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what affects primer specificity? - (reply: 2)
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Primers PCR - storage (reply: 5)
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Human Epigenome Project primers? - (reply: 2)
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Degenerate Primers - Help with Degenerate Primers (reply: 1)
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ready to use primers for BSP - (reply: 4)
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what's the difference between sequencing primer and cloning one. - confused (reply: 5)
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GAPDH primer sequences for mRNA level in HeLa cells - (reply: 2)
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FGF20 PRIMER - (reply: 2)
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primer design for Syber Green - (reply: 1)
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primer walking design tool - (reply: 1)
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primer design for MSP and BSP - primers must be in promoter region? (reply: 1)
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Degenerate Primers and dI - (reply: 5)
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designing a primer - (reply: 5)
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using primers programms - (reply: 9)
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PCR Trouble Shooting - new primer, wrong dilution of primers, wrong band, and many more (reply: 15)
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long range PCR primer design - (reply: 1)
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what is CDS primer used as a control for QPCR in CHIP? - (reply: 2)
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How to get rid of primer dimers - (reply: 2)
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one nucleotide in the primer is wrong - (reply: 2)
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real time PCR using two sets of primers with different melt curves - (reply: 4)
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Introduce restriction sites with PCR primers - (reply: 2)
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MSP Primer design -rules to choose primers and reasons - (reply: 2)
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Primer calculation - (reply: 3)
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Testing newly designed primers prior to real time - (reply: 2)
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information regarding PCR using very long primers - (reply: 4)
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Bisulfite: PCR condition or primer problem? - (reply: 13)
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storage of primers - (reply: 3)
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too many hits on Blast for primers / microsatellite - (reply: 4)
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sequencing new "unknown" plasmid - any good SV40 primers out there? (reply: 1)
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Primer orientation, forward and reverse - (reply: 4)
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Negative control primer for ChIP - (reply: 3)
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RT-PCR primer designing - (reply: 6)
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Free program for primer designing? - Need to check promoter sequence for possible primer binding sites (reply: 3)
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My primers don't work with Chip - (reply: 1)
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qPCR using random primers - (reply: 4)
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Lambda DNA nanowires, Restriction sites and thiolated primers - (reply: 1)
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Amplifying a 1700bp fragment using a mismatched primer pair - (reply: 2)
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Degenerate Primers - (reply: 12)
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Plasmid DNA Sequencing with Universal Primers - (reply: 3)
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Optimisation of dCAPS markers (degenerate primers) - problems with detection of heterozygotes (reply: 1)
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Possible mutations in primers? - (reply: 4)
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Need to confirm PCR primers are bad - (reply: 5)
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Help! Primer Dimers… - (reply: 3)
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Very low PCR primer concentrations? - (reply: 4)
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Primer sets for Bisulfite-modified and unmodified DNA - (reply: 6)
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Primer Design for Gene Inserted Counterclockwise into Plasmid - Please help...very basic question for a mol. biologist (reply: 1)
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False priming in MSP - (reply: 4)
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Can primers go bad and give a smear in PCR? - (reply: 12)
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QuickChange primers self-made - (reply: 4)
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PCR Conditions for primers with GC Clamp and without GC Clamp - (reply: 3)
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digoxygenin-labelled primer - (reply: 3)
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freezing of primers - (reply: 2)
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Primer dilution and calculation - (reply: 4)
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Designing primer for Banana mild mosaic virus - (reply: 3)
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primer for real-time and reverse transcriptase PCR - (reply: 1)
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Bisulfte PCR and sequencing PCR and Primer Notes - Tips and hints on PCR for bisulfite converted DNA and bisulfite primer (reply: 33)
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humanized antibody - how to design primers for CDRs - humanized antibody (reply: 1)
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5' Primer modification - Tm-effect ?? (reply: 1)
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sequencing - which primer should I trust? (reply: 4)
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Help- degenerate primer design - (reply: 4)
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Use of W primers to detect efficiency of bisulfite reactions? - (reply: 5)
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Is a Kazark sequence needed in a upper primer? - (reply: 17)
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cytokines primers - (reply: 1)
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Inverse PCR primers design - (reply: 4)
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general pcr/primer question - (reply: 1)
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primers in PCR - primer removed or not (reply: 5)
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different primer efficiency using cDNA and plasmid/PCR product - (reply: 4)
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Primers for BS treated DNA - (reply: 5)
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concentration of primers - (reply: 5)
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forward & reverse primers - (reply: 3)
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Primers & probes of Applied Biosystems for real time - (reply: 2)
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Methyl Primer Express from ABI - (reply: 2)
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design primer - (reply: 5)
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can Primers for RT-PCR be used Real-Time PCR directly? - (reply: 2)
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primer design questions for confused - (reply: 2)
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primer selection for RT prior to real time - an insiders hint!!! (reply: 1)
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primer concentration - (reply: 7)
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RT-PCR primer design: Over introns and exons? - (reply: 2)
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SYBR VS TAqman- What is the difference other than PRimers? - TAqman works and SYBR doesnt (reply: 1)
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pcr yield and primer concentration - (reply: 5)
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Primer design with restriction sites - (reply: 3)
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differences between oligo and random primers - (reply: 2)
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Any primer design programs...? - (reply: 8)
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Cutting out of a T vector - Cutting sites in primers vs sites in vector (reply: 3)
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primer : shifting peak - (reply: 4)
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Designing primers and hybridization probes - (reply: 1)
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ChIP primer for promoter region of GAPDH - for taqman analysis (reply: 2)
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Methylation specific primer designing - (reply: 1)
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Primer Extension Assay versus RACE-PCR - Protocol (reply: 1)
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RT-PCR primer design - (reply: 1)
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how to check primers - (reply: 4)
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TM for primers with restriction secuence tail. - Suggestion (reply: 3)
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How to determine PCR Annealing temperature for primers with different Tm - (reply: 7)
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PCR with 75 mer forward primer - (reply: 4)
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about the primers in MSP - (reply: 5)
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primer dimer in pcr product - cloning for a gene. analyzing pcr product shows primer dimer (reply: 8)
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PCR, labelled vs unlabelled primers - (reply: 4)
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Using pentadecamer primers for reverse transcription - Better than hexamers, oligo d(T)? (reply: 7)
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Bands appear poor/not at all and do not appear consistantly & primer bands a - (reply: 2)
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The weirdest primer ever - (reply: 7)
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no RT controls - can I run on separate plate and for just one primer set? (reply: 1)
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What happens to PRIMERS at 72 C ? - (reply: 5)
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Melting Curve giving flutating Ct values - Advice on primer design? (reply: 9)
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Direct BSP using tag-modified primers - (reply: 2)
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Mutagenesis using 4 sets of primer - (reply: 6)
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PCR analysis of clones - Can a gene specific primer pair amplify a fragment in the wrong direction? (reply: 1)
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pro-PCR with only one primer... - to check directionality. (reply: 4)
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cDNA synthesis primer - (reply: 3)
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antigen primers - (reply: 1)
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Annealing vs melting Ts for real time PCR primers - (reply: 1)
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Calculating Tm for bisulfite primers? - (reply: 3)
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Using primers meant for DIG probe as PCR diagnostic - (reply: 1)
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Need for Nested PCR - Primers Designed with MethPrimer - (reply: 6)
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Primer for 5' RACE - (reply: 2)
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ABI have released MethylPrimer Express! - A new primer design program for DNA methylation....For Free!!! (reply: 45)
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PCR using long primers and two overlapping templates - PCR using long primers and two overlapping templates (reply: 2)
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PCR primers with high Tm - (reply: 6)
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Primer Dimer problem- Different perspective - COuld there be a treatment effect (reply: 4)
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making primers for similar protein sequences - making primers for similar protein sequences (reply: 4)
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Bisulfite sequencing primer - (reply: 4)
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random primers for qPCR - (reply: 3)
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difficulty in Primer walking - (reply: 2)
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Primer for sequencing - need help!!!!!!!!!! (reply: 6)
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Primer design invert pcr - (reply: 5)
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Vector construction - Can I use long PCR primers (60bp) and get good PCR poduct??? (reply: 4)
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Stem loop RT-PCR for miRNA quantification - how can I design the stem loop primer? (reply: 2)
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BSP Primer placement over KNOWN unmethylated CpG's - Can this be done? (reply: 3)
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dimers of dna (not primers) following digest or pcr? - (reply: 11)
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Question about Realtime PCR primer concentration.... - (reply: 5)
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plasmid amplification - exons and primers (reply: 1)
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reverse primer design with restriction site - (reply: 6)
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question in 5' RACE and primer extesion - (reply: 8)
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PCR product dimer (not primer dimer!) - PCR (reply: 2)
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from which strand to derive BSP primers? - (reply: 5)
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Primer Annealing for PCR - (reply: 4)
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Site-Directed Mutagenesis- Doubt - Overlapping primers (reply: 3)
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How to design primer for sequencing unknown DNA - (reply: 5)
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need helps on probe design--human ip10 primers and probe. - (reply: 2)
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Sequencing Primers - (reply: 4)
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RT PCR on ALM patients - help for designing primers for detect PML RAR (reply: 2)
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Primer design with incomplete sequence - (reply: 2)
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PCR primers from amino acids? - (reply: 1)
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primers design for mutagenesis of two nearby sites - mutations (reply: 2)
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Intensive primer dimers - (reply: 2)
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PCR is positive with one set of primers, negative with another. - (reply: 4)
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Trouble with primers of BSP - (reply: 3)
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potassium acetate in primer solution - I made a mess but need to recover (reply: 6)
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primer design - how to design primers (reply: 2)
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Why primers for bisulfite converted DNA amplify unconverted DNA? - (reply: 3)
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Reverse transcription of poly-A tailed miRNAs using anchored Oligo dT primers? - (reply: 6)
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southern hybridizaion with 5' end labelled primer - (reply: 1)
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Disadvantages of using oligo-dT primers - (reply: 1)
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do primers and dNTPs alter digestion efficiency? - (reply: 4)
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PCR with degenerate primer - (reply: 6)
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RT-PCR primers for E. coli LacZ gene - (reply: 3)
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primer reconstitution - (reply: 2)
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Primer for 4 genes sharing short consensus sequence - (reply: 2)
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Looking for online primer design for SNaPshot - (reply: 1)
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PCR primers: hairpin and dimers? - what can I do to avoid this? (reply: 4)
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multiplex PCR for three sets of primers - (reply: 1)
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How many mismatches in point mutation primers? - (reply: 3)
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single primer PCR - use a single primer to amplify regions between inverted repeats (reply: 1)
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Mouse MSP primers - (reply: 11)
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Primer Dimer problems in real-time PCR - (reply: 14)
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Degenerate primers for nested PCR? - (reply: 1)
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Reliable ChIP primers for Sp1? - (reply: 2)
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increased primer dimer with template - (reply: 2)
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free software to examine if two primers will make dimers - (reply: 3)
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Testing primers - (reply: 3)
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Two round MSP with the same primer - (reply: 1)
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Designing a Primer within an exon - Consesus required - (reply: 2)
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difference between primer extension and RACE - (reply: 6)
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Primer - (reply: 1)
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rat 18s rRNA primer - designing primers (reply: 1)
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Designing primers for multiplex - BLASTing lots of primers (reply: 3)
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Primer design question - Use of primer 3 (reply: 3)
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RT-PCR Primer design - which region? - (reply: 2)
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No PCR products in MSP with MethPrimer designed primers - (reply: 2)
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design primer for PCR.. - (reply: 3)
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Design primer - (reply: 2)
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extra sequence in primers - (reply: 4)
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calculating Tm of primers - (reply: 8)
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How to design random primers - (reply: 2)
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PCR primers for Mycoplasma detection? - Publication gives many, which to choose? (reply: 1)
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primer design problem - (reply: 1)
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60 bp PCR and primer dimers - (reply: 6)
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Primer Express Software - Probe Design (reply: 8)
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Problems with methylation analysis!!! - Primers crossreaction etc. (reply: 3)
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designing flanking primers around msp primers - (reply: 1)
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BSP cycles and primers - Is it OK to use the same primer set but with more (reply: 6)
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primer does not bind - help (reply: 2)
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SOS call: ChIP assay in Huh7 cells - How many cells? And primer position? (reply: 3)
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RT-PCR inconsistent primer efficiencies - RT-PCR (reply: 3)
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is it effective to use a primer of SD rat to Wistar rat in PCR - help (reply: 2)
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Primers for RT reaction - (reply: 6)
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How to prepare primer solution - (reply: 7)
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primer storage - (reply: 7)
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Help: Check my MSP primers - is my MSP primer correct??? (reply: 1)
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reducing primer dimer - (reply: 1)
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primer melting temperature - (reply: 2)
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end of primer - (reply: 2)
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PCR primer dimer? - (reply: 7)
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PCR primer Tm and concentration question - (reply: 3)
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cloning primer orientation - orientation and complement of primers in a cloning (reply: 3)
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primer design - checking off-target site - (reply: 5)
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Which RT primer is better for qPCR - (reply: 2)
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Why different amounts of forward and reverese primers in RT-QPCR - (reply: 2)
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primer optimization - (reply: 1)
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multiplex PCR - template and primer concentration (reply: 2)
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Real Time qPCR - qPCR SYBR greenI primer design (reply: 5)
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The perfect number of CpGs in MSP primer - (reply: 8)
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Primers - (reply: 1)
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Probes or primers that span introns - (reply: 6)
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what primers specific for B.stearothermophilus?Help pls. - (reply: 5)
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problem with my PCR - primer dimer. - (reply: 1)
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how long a primer should I use? - (reply: 1)
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what is the "tailed primer and taq primer"? - (reply: 2)
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Difficutly go separate product from primer dimers - Methylation specific PCR (reply: 1)
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designing gene-specific primer for cDNA synthesis - (reply: 5)
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How to design primers for CpG, CpNpG, CpHpH methylation - Bisulfite Sequencing PCR (reply: 2)
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software to find restriction site and TM for primer - (reply: 7)
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product size for real-time PCR primer - (reply: 3)
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real time PCR primers - (reply: 2)
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cpgware primer design, does it work - (reply: 4)
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PCR Primer Design with restriction sites - (reply: 1)
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Sequence input for bisulfite PCR primer design - (reply: 1)
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Database software to catalogue / archive primers - Database software to catalogue / archive primers (reply: 1)
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primer design for bisulphite treated DNA - (reply: 1)
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primer design: end-point vs. RT - (reply: 1)
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software to check the primer dimer - (reply: 3)
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BSP and MSP primer check - (reply: 2)
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How to get rid of PCR primer dimer - (reply: 65)
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RT-PCR primer design - exon-exon primer design (reply: 5)
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availabre primers for MSP - (reply: 1)
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RACE - specific primers in first strand synthesis (reply: 4)
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Primer Designer Online - Pipleline use of designer (reply: 1)
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methylation primers - problems with primers (reply: 1)
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real-time PCR - How to design primers (reply: 2)
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PCR primers - PCR primers for RT-PCR (reply: 1)
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Primers: advice? - (reply: 1)
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bisulfite pcr primers - (reply: 1)
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RT-PCR primer design guide - How to check gene structure and design the primer? (reply: 15)
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Double strand cDNA sysnthesis - Using random primers (reply: 1)
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Need suggestions for degenerate primers - (reply: 2)
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Primer design - Primer design for RT-PCR (reply: 1)
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Primer contamination - (reply: 2)
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questions about degenerate primer PCR - (reply: 2)
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How to find/check the highly conservative sequence - RT-PCR primers (reply: 2)
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primer purification - search method for primer purifying (reply: 3)
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Software for MSP (methylation-specific PCR) primer design - (reply: 3)
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