Protocol Online logo
Top : Forum Archives: :
Culture Related Discussions
  1. DRG Culture - (reply: 1)
  2. Overnight culture - (reply: 1)
  3. Contamination and its fungus - persistant fungal contamination in cell cultures (reply: 1)
  4. how to start cord blood culture - (reply: 1)
  5. can you transform directly into liquid culture? - (reply: 2)
  6. please help me identify if I have cell culture contamination =( - (reply: 1)
  7. Bacterial culture - (reply: 8)
  8. Weird contaminant in culture? pics included - unknown contaminant (reply: 7)
  9. Changing the pH of liquid e.Coli cultures. - (reply: 5)
  10. Preservation of Bacterial Culture - (reply: 7)
  11. ATDC5 CELLS CULTURE PROBLEMS! - (reply: 3)
  12. how to purify the primary preadipocyte culture? - many fibroblast contamination (reply: 4)
  13. Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
  14. cell culture - (reply: 1)
  15. Inhibitors in cell culture... - DMSO???? (reply: 4)
  16. cell culture - (reply: 1)
  17. Strawberry Tissue culture - (reply: 1)
  18. cell culture protocol (URGENT) - (reply: 1)
  19. Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
  20. tobacco tissue culture - (reply: 2)
  21. fuzzy balls in my culture! - Help (reply: 1)
  22. Bacterial culture in microcentrifuge tube? - Staphylococcus (reply: 8)
  23. Culture supply - (reply: 2)
  24. Pichia culture in flask - cover with cheese cloth or gauze? (reply: 6)
  25. How to culture Top10 with pBI121 ? - (reply: 1)
  26. What solvent should i use to dissolve a herbal extract - difficulty in dissolving lyophilised herbal extract for use in culture (reply: 3)
  27. How long can a bacterial culture be storage be stored at 4 degrees? - (reply: 12)
  28. Culture media with human serum gives false positive - (reply: 1)
  29. Why 10% serum for cell culture? - (reply: 11)
  30. Plasmid lost during culture - (reply: 19)
  31. Cell culture contamination - (reply: 9)
  32. cells culture - (reply: 6)
  33. clear culture, but PCR positive - (reply: 4)
  34. difference between subculture in incubator and shaker - on preparation of cultures for plasmid dna extraction, is there a diff (reply: 3)
  35. Hybridoma cultures - floaters? - (reply: 4)
  36. Culture of Epithelial cells from Breast Milk? - (reply: 2)
  37. Striatal and/or SN neuronal primary culture - (reply: 2)
  38. Magnetic stirr bars - cell culture (reply: 3)
  39. culture of spleen cell - (reply: 2)
  40. cell line culture - (reply: 2)
  41. Unexplained Cell Culture Death - Expertise needed (reply: 7)
  42. Elisa for total human IgG from culture supernatant - (reply: 1)
  43. Cell Culture Question - (reply: 3)
  44. how to culture mouse MSCs from bone marrow - (reply: 1)
  45. Observed tiny black dots on cell culture - (reply: 9)
  46. anyone culture SKOV3 cells? what's the morphology under microscope? - (reply: 3)
  47. Monocyte culture - How to get the monocytes to adhere to culture plates? (reply: 3)
  48. Bacteria culture protocols - (reply: 4)
  49. is a matrigel layer a 3d cell culture? - (reply: 6)
  50. Hybridoma culture not secreting mouse antibody - (reply: 6)
  51. Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
  52. Media turns Turbid after 3 days of culture - (reply: 6)
  53. contamination of my culture? - (reply: 3)
  54. THP-1 cell culture problem: Cells die after thawing - (reply: 4)
  55. Cell culture using laminin - (reply: 1)
  56. Differentiating cultures between commensal and pathogenic - (reply: 2)
  57. Best Guess:How many cells in Organotypic Slice culture - (reply: 2)
  58. How to prepare conditioned media? - Do I have to omit supplements for the culture media? (reply: 2)
  59. DMSO in cell culture - is it toxic, does it change signalling? - Citations wellcommed (reply: 2)
  60. how to separate dead from viable cells in cultures of non-adherent cells - (reply: 3)
  61. THP-1 cells culture - (reply: 4)
  62. Doesn't anyone use roller bottles to culture cells in anymore? - Let's compare notes. (reply: 2)
  63. How much antobody to bind to bacterial culture? - (reply: 1)
  64. Smooth muscle cell culture - (reply: 4)
  65. Contamination or not? - Cell Culture Contamination (reply: 1)
  66. passage of cell culture and protein locolization - (reply: 1)
  67. culture CD34+ Hematopoietic Stem Cells - (reply: 2)
  68. How much serum should I put in the tissue culture - (reply: 5)
  69. human serum in cell culture - is it possible to use human serum in cell culture? (reply: 14)
  70. Who makes 200-350cm2 tissue culture roller bottles? - (reply: 8)
  71. Tissue culturing - Crash down of culture -Unknown reason!! (reply: 4)
  72. How do I culture adherent TZM-bl cells?! - (reply: 5)
  73. help! cell culture contamination too! - (reply: 5)
  74. Sodium pyruvate in ES cell culture media - (reply: 2)
  75. microbial contaminant in plant tissue culture...?! - (reply: 1)
  76. primary colonic epithelial cell culture - (reply: 1)
  77. Suspension culture - (reply: 1)
  78. pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
  79. cell culture contamination - after transfection (reply: 15)
  80. culture identification - (reply: 3)
  81. Basic CHO cell culture - (reply: 3)
  82. Alternative Sera for Cell Culture - (reply: 6)
  83. Cell culture sterility Checks (DMEM) - (reply: 3)
  84. Immunoprecipitation of culture medium - (reply: 4)
  85. Loss of RNAi after changing culture media? - (reply: 2)
  86. Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
  87. problems about THP-1 cell culture - (reply: 1)
  88. cell culture with serum media and with serum free media and doubts in luciferase - (reply: 1)
  89. Can cell culture media be frozen at -20°C - (reply: 4)
  90. diluting 2x10e12 culture to 4x10e10 etc? - (reply: 1)
  91. what affects cell culture viabilty? - (reply: 8)
  92. Neurosphere culture - (reply: 2)
  93. Stock culture - (reply: 4)
  94. Culture of rat cortical neurons - E16 or E18? (reply: 14)
  95. B27/Neurobasal medium for hippocampal neuron culture - (reply: 7)
  96. mouse fibroblast culture: what medium? - (reply: 7)
  97. Assaying apoptosis on non-detaching cell culture plates - which plates? (reply: 1)
  98. need help for cord blood mesenchymal stem cell culture - (reply: 6)
  99. Culture based technique - (reply: 1)
  100. Help! Large vacuoles observed in HEPG2 cell culture! - (reply: 3)
  101. isolation of small RNAs from culture supernatant - (reply: 11)
  102. how important of Serum proportion of culture medium in cell culture - SOS (reply: 8)
  103. E.coli culture and miniprep help - (reply: 1)
  104. Culture medium for MDA-MB-231 / MCF-7 - which media is good enough? (reply: 6)
  105. cell culture - a beginner - (reply: 3)
  106. Measuring proliferation via cell culture medium - is it possible? (reply: 2)
  107. Overnight culture methods - (reply: 5)
  108. Confocal microscopy with primary cell cultures - (reply: 8)
  109. Subcultures of primary cultures - would you use them for your experiments? - (reply: 3)
  110. How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
  111. lymphocyte culture - problem in isolating lymphocytes from spleen culture (reply: 1)
  112. Culture Confluency - (reply: 4)
  113. Remove BSA from cell culture medium - (reply: 5)
  114. Establishment of primary fibroblast culture - (reply: 3)
  115. Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
  116. Basics of Cell Culture - (reply: 2)
  117. Primary Myoblast Culture - Could u help me in myoblast culture and differention (reply: 1)
  118. Filtration of bacterial culture - (reply: 1)
  119. rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
  120. Trouble going from plates to liquid culture - (reply: 2)
  121. Higher biomass yield in batch cultures - (reply: 1)
  122. fiborblast contamination in primary culture - (reply: 7)
  123. Cell Culture Book - any suggestion? (reply: 29)
  124. cell culture - (reply: 2)
  125. Thp-1 cell culture problems - (reply: 5)
  126. Cell Culture with Human Plasma - (reply: 2)
  127. rat's umbilical cord culture - (reply: 4)
  128. How to limit fibroblast cell contamination in epithelial cell culture - (reply: 9)
  129. can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
  130. culture blood vessel vitro - (reply: 2)
  131. cell culture western blot - (reply: 15)
  132. Can adherent culture be transformed into suspension cells? - (reply: 4)
  133. human bone marrow msc culture - (reply: 2)
  134. E.Coli culture contamination 2 - (reply: 10)
  135. Tetracycline in cell culture media - (reply: 5)
  136. Loss of cell viability in primary culture - (reply: 4)
  137. Question on bacterial culture - (reply: 5)
  138. Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
  139. Why tumor cells are hard to culture - (reply: 3)
  140. T cell culture - ag specific T cell culture (reply: 1)
  141. What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
  142. Immunofluorescence of frozen cell culture inserts - (reply: 6)
  143. How to avoid contamination in tissue culture? - (reply: 11)
  144. primary cell culture death - (reply: 8)
  145. ELISA IL12 p70 from mouse dendritic cells culture SN - (reply: 2)
  146. new lab, cells die after passaging - general fish cell culture methods (reply: 5)
  147. mouse spleen cell culture - (reply: 2)
  148. Has any one successfully decontaminated a contaminated cell culture - (reply: 3)
  149. culture of synovial fibroblasts (mouse) - (reply: 1)
  150. Why HepG2 and HUH7 - for hepatitis cell culture matter. (reply: 3)
  151. cell culture contamination - (reply: 2)
  152. Differential media neuronal culture - (reply: 1)
  153. Culture of adherent SH-Sy5y Neuroblastoma Cell Line - (reply: 5)
  154. Dissociated neuronal cultures - (reply: 2)
  155. microglia cell culture - (reply: 5)
  156. Setting up a Cytoxicity Assay - cell culture (reply: 1)
  157. Cell Biologists working in 3d cell cultures - (reply: 1)
  158. Use of antibiotics in cell culture? - (reply: 5)
  159. Is myc endogenously expressed in primary hippocampal neuronal cultures? - positive myc immunostaining in hippocampal cultures (reply: 1)
  160. Aspergillus niger culture media - (reply: 3)
  161. Good book on cell culture - (reply: 5)
  162. macrophages culture, please help - (reply: 1)
  163. Unknown culture contamination? - (reply: 7)
  164. Maize tissue culture - (reply: 5)
  165. What's the maximum amount of DMSO you can use in cell culture? - (reply: 4)
  166. desert plants - for tissue culture (reply: 6)
  167. about culture temperature - (reply: 5)
  168. probles when culture 293T culture? - (reply: 6)
  169. getting rid of shaky hands during cell culture/pipetting - (reply: 9)
  170. somaclonal variation in tissue culture - (reply: 4)
  171. Splenocyte culture - Cell death upon in vitro culture (reply: 1)
  172. When air bubbles is not encouraged in tissue culture? - (reply: 4)
  173. arabidopsis leaf tissue culture - (reply: 3)
  174. why overnight culture is necessary - (reply: 3)
  175. Primary culture hepatocytes of mouse - (reply: 2)
  176. possible contamination of a cell culture? - (reply: 3)
  177. Cornea Epithelial Cell culture - (reply: 1)
  178. Plant tissue culture - (reply: 1)
  179. questions about bacteria culture medium - (reply: 1)
  180. Plant tissue culture - (reply: 7)
  181. how to culture primary neuron in coated coverslip - how to culture primary neuron in coated coverslip (reply: 5)
  182. Will the addition of CO2 to cultures boost cyanobacterial growth rate? - (reply: 2)
  183. general cell culture (and IMR90 line) - any resource to find out info about lines (reply: 1)
  184. Re-innoculate overnight culture for miniprep - (reply: 4)
  185. Isolation and culture of white blood cells. - (reply: 1)
  186. Paraformaldehyde detaches my 3T3 culture - (reply: 2)
  187. need help for culture protacal of new stem cell - (reply: 5)
  188. Where to Obtain Pure Algal Cultures - (reply: 3)
  189. puromycin in culture medium - (reply: 3)
  190. Growing bacteria for several cultures - (reply: 6)
  191. Culture problems of CRL-2331 cells (ATCC) - (reply: 3)
  192. Problems with Ba/F3 cell culture - (reply: 1)
  193. Water or TSB to dilute an over night culture!? - (reply: 6)
  194. Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy? (reply: 2)
  195. culture maintainence - bacterial culture maintainence (reply: 1)
  196. How to culture rat dorsal root ganglia cell? - (reply: 1)
  197. tissue culture Garlic/Onion - protocol (reply: 4)
  198. Primary culture of breast tumour - Does anyone know the tissue slice method? (reply: 3)
  199. Primary culture - keratinocytes - how do I know? (reply: 6)
  200. Cytokine/chemokine detection in culture supernatant - (reply: 6)
  201. keratinocyte culture media - (reply: 8)
  202. Help with endothelial cells - Endothelial Cells Culture (reply: 4)
  203. DRG cell culture - (reply: 6)
  204. culture media left out for too long? - (reply: 7)
  205. Serial dilution and quantitative culture of S. aureus - (reply: 4)
  206. lncap cell culture - (reply: 3)
  207. collagen-coated culture dishes - How to make? (reply: 14)
  208. mouse embryonic cortical organotypic cultures - (reply: 1)
  209. HL60 in culture and differentiation - (reply: 1)
  210. pH balance - HCO3/CO2 buffer in culture media (reply: 1)
  211. Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
  212. Sphere cells suspension culture - (reply: 2)
  213. HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
  214. 30 degrees versus 37 degrees for E coli culture - (reply: 7)
  215. Websites or books about stem cell culture - (reply: 2)
  216. Help with concentrating proteins in culture medium - (reply: 4)
  217. primary culture - measuring transcription - mRNA expression in primary culture (reply: 1)
  218. Adenovirus culture/isolation - (reply: 3)
  219. help:how to culture mouse embryonic neuron(E13.5) ? - (reply: 2)
  220. Organ Culture - (reply: 4)
  221. questions about mass spectrometry and cell culture - (reply: 1)
  222. Separation of dead cells from cultures - (reply: 8)
  223. Function of beta-mercaptoethanol in cell culture medium - (reply: 6)
  224. Black Debris in T-Cells - debris in my cell culture (reply: 3)
  225. dendritic cell isolation - cell culture (reply: 1)
  226. Tissue culture of algea - (reply: 3)
  227. Primary Culture of keratinocytes - How to start a keratinocyte culture (reply: 4)
  228. Keratinocyte cultures with serum or serum free media - (reply: 1)
  229. Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
  230. Preparing cultures of know concentration - How to do it? (reply: 5)
  231. Concentration of secreted protein in culture media - Concentration of culture media (reply: 7)
  232. coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
  233. Macrophage culture problems! please help! - macrophages (reply: 1)
  234. detecting Mycoplasma in cultures - (reply: 7)
  235. Do you reuse cell culture vessels? - (reply: 3)
  236. 100mm culture dish - (reply: 5)
  237. overnight culture - (reply: 3)
  238. Ingredient for keratinocyte culture medium - (reply: 1)
  239. Shaker rotation to grow bacterial cultures? - (reply: 8)
  240. human rTGFß for mouse cell culture applications? - (reply: 1)
  241. 50% stock cultures - (reply: 2)
  242. Tissue culture and cell culture? - (reply: 4)
  243. Ventation of culture vessles - (reply: 5)
  244. MDA-MB-231 cell culture - (reply: 7)
  245. Filtration of primary cell culture, how?? - (reply: 2)
  246. filtering for cell culture stuff - filter cell culture (reply: 5)
  247. tgf-beta in cell culture - (reply: 1)
  248. Mouse pancreatic primary culture: protocol is needed - (reply: 4)
  249. Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
  250. primary keratinocytes - HeKn - problem with culture (reply: 2)
  251. Sterilizing a Cell Culture Room - We had a flood + mold, where do I start? (reply: 1)
  252. E. coli culture contamination - (reply: 2)
  253. Contamination issue in cell culture - (reply: 109)
  254. non-enzymatic dissociation - cell culture (reply: 1)
  255. Freezer culture fails to retain plasmid - (reply: 3)
  256. Ht-29 Subculture - Cell culture (reply: 1)
  257. tissue culture of jatropha - tissue culture of biodiesel producing plant (reply: 22)
  258. preventing infection of cell cultures - (reply: 3)
  259. anaerobic bacteria - how to stock culture (reply: 2)
  260. help: primary endothelial cell culture! - (reply: 3)
  261. Myofibroblast Culture - (reply: 1)
  262. concentrate protein in culture media? - (reply: 2)
  263. 4 layers instead of a monolayer in cell culture - (reply: 2)
  264. Plant Culture Cell - (reply: 1)
  265. Serum in Cell Culture - Use of FBS etc. in cell culture (reply: 3)
  266. Monocyte culture - (reply: 5)
  267. how to culture a cell line of "fibroblast" - (reply: 5)
  268. Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
  269. Endothelial cells culture - Protocols for the culture of endothelial cells, special tips (reply: 2)
  270. Formalin fixation&paraffin-embedding of tissue culture cells - Formalin fixation&paraffin-embedding of tissue culture cells (reply: 1)
  271. animal cell culture - (reply: 2)
  272. Endotoxin in culture media - Endotoxin in culture media (reply: 2)
  273. Macrophage culture - (reply: 4)
  274. How to concentrate protein from culture medium - for Western blot (reply: 1)
  275. lymphocytes - help wanted for culture (reply: 1)