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Culture Related Discussions
     
          
          
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                            DMEM Medium - suspension culture/adherent culture (reply: 1)
 
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                            DRG Culture -  (reply: 1)
 
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                            Changing Media for Lentiviral Infection in a Suspension Culture -  (reply: 1)
 
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                            Overnight culture -  (reply: 1)
 
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                            THP-1 cell culture -  (reply: 3)
 
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                            Contamination and its fungus - persistant fungal contamination in cell cultures (reply: 1)
 
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                            cell culture maintainance -  (reply: 3)
 
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                            how to start cord blood culture -  (reply: 1)
 
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                            trypsin - wat is the source of trypsin we use in cell culture (reply: 1)
 
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                            can you transform directly into liquid culture? -  (reply: 2)
 
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                            RNA Extaction from microglial cells culture - the best method for RNA extraction of microglial cells culture (reply: 2)
 
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                            please help me identify if I have cell culture contamination =( -  (reply: 1)
 
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                            microplate for cell culture? -  (reply: 1)
 
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                            Bacterial culture -  (reply: 8)
 
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                            identification of cell culture contamination -  (reply: 1)
 
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                            Weird contaminant in culture? pics included - unknown contaminant (reply: 7)
 
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                            I need help for my Raji cell culture -  (reply: 2)
 
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                            Changing the pH of liquid e.Coli cultures. -  (reply: 5)
 
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                            cell culture question - passage number -  (reply: 5)
 
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                            Preservation of Bacterial Culture -  (reply: 7)
 
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                            how to transport a culture cell 35 mm plate or 25cm2 flask - confluence is very very slow (reply: 3)
 
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                            ATDC5 CELLS CULTURE PROBLEMS! -  (reply: 3)
 
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                            strange bacteria culture -  (reply: 3)
 
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                            how to purify the primary preadipocyte culture? - many fibroblast contamination (reply: 4)
 
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                            Different cell culture plastics, problems with cell adherance -  (reply: 6)
 
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                            Cell culture supernatant - Is it just the media from cultured cells? (reply: 1)
 
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                            Pseudomonas aeruginosa - difficult to culture? - and other questions (reply: 3)
 
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                            cell culture -  (reply: 1)
 
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                            Quenching oxygen in cell culture -  (reply: 1)
 
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                            Inhibitors in cell culture... - DMSO???? (reply: 4)
 
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                            Neuronal cultures on polyornithine coated glass coverslips -  (reply: 4)
 
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                            cell culture -  (reply: 1)
 
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                            Sample collection from culture of facultative anaerobic bacteria growing anaerob -  (reply: 5)
 
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                            Strawberry Tissue culture -  (reply: 1)
 
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                            Cell Culture protocol by Current Protocol - free online version from invitrogen (reply: 3)
 
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                            cell culture protocol (URGENT) -  (reply: 1)
 
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                            PMA Ionomycin Cell Culture / Dilution and Math Check -  (reply: 1)
 
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                            Cell culture being poisoined for nearly a year - Done by unknown person(s) (reply: 33)
 
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                            what protease is best for primary cell culture -  (reply: 1)
 
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                            tobacco tissue culture -  (reply: 2)
 
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                            3D culture keratinocytes- recommendations? -  (reply: 2)
 
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                            fuzzy balls in my culture! - Help (reply: 1)
 
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                            methylated plasmids in culture -  (reply: 1)
 
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                            Bacterial culture in microcentrifuge tube? - Staphylococcus (reply: 8)
 
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                            Mycoplasma - How to detect and treat mycoplasma contamination in culture and RNA? (reply: 9)
 
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                            Culture supply -  (reply: 2)
 
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                            how to culture cancer stem cells? - help (reply: 1)
 
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                            Pichia culture in flask - cover with cheese cloth or gauze? (reply: 6)
 
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                            Question on culture expiration -  (reply: 4)
 
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                            How to culture Top10 with pBI121 ? -  (reply: 1)
 
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                            Primary tracheal cell culture -  (reply: 1)
 
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                            What solvent should i use to dissolve a herbal extract - difficulty in dissolving lyophilised herbal extract for use in culture (reply: 3)
 
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                            What solvent should i use to dissolve a herbal extract - difficulty in dissolving lyophilised herbal extract for use in culture (reply: 5)
 
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                            How long can a bacterial culture be storage be stored at 4 degrees? -  (reply: 12)
 
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                            Please help with E.coli culture! -  (reply: 4)
 
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                            Culture media with human serum gives false positive -  (reply: 1)
 
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                            Can I load 1N NaOH in cell culture plate? - 1N NaOH is strong alkaline, will it eat up the glass? (reply: 4)
 
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                            Why 10% serum for cell culture? -  (reply: 11)
 
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                            Cell culture basic questions - Basic questions on cell culture (reply: 7)
 
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                            Plasmid lost during culture -  (reply: 19)
 
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                            Leakage of ER(T2)-fusion protein ? - in cell culture (reply: 2)
 
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                            Cell culture contamination -  (reply: 9)
 
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                            What is needed for a hybridoma growth culture -  (reply: 1)
 
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                            cells culture -  (reply: 6)
 
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                            I thawed my primary cells and froze them again? serious mistake? - cell culture (reply: 5)
 
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                            clear culture, but PCR positive -  (reply: 4)
 
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                            Cell culture unidentifiable 'contaminant' - weird things appearing in my culture (reply: 1)
 
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                            difference between subculture in incubator and shaker - on preparation of cultures for plasmid dna extraction, is there a diff (reply: 3)
 
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                            How to get rid of contamination in human PBMC culture? - Been having problem in culturing human PBMC (reply: 5)
 
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                            Hybridoma cultures - floaters? -  (reply: 4)
 
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                            How much media needed for cells in different cell culture plates -  (reply: 3)
 
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                            Culture of Epithelial cells from Breast Milk? -  (reply: 2)
 
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                            Cell clumping in culture: Folic acid or i-Inositol? -  (reply: 3)
 
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                            Striatal and/or SN neuronal primary culture -  (reply: 2)
 
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                            Rescuing contaminated S. cerevisiae culture with antibiotics? -  (reply: 1)
 
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                            Magnetic stirr bars - cell culture (reply: 3)
 
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                            MEF culture -  (reply: 4)
 
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                            culture of spleen cell -  (reply: 2)
 
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                            PBS with or without Calcium and Magnesium - When it is important to use either one in Cell culture?? (reply: 2)
 
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                            cell line culture -  (reply: 2)
 
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                            Clumping and contamination in cell culture -  (reply: 2)
 
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                            Unexplained Cell Culture Death - Expertise needed (reply: 7)
 
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                            How to do this technique using culture procedures? -  (reply: 4)
 
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                            Elisa for total human IgG from culture supernatant -  (reply: 1)
 
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                            New to Cell Culture and need some basic information - help needed (reply: 12)
 
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                            Cell Culture Question -  (reply: 3)
 
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                            what kind of contamination is this? (photos inside) - contamination in cell culture with HeLa (reply: 10)
 
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                            how to culture mouse MSCs from bone marrow -  (reply: 1)
 
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                            RAW mouse macrophages floating in culture -  (reply: 2)
 
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                            Observed tiny black dots on cell culture -  (reply: 9)
 
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                            RNA extraction from plant cell cultures -  (reply: 2)
 
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                            anyone culture SKOV3 cells? what's the morphology under microscope? -  (reply: 3)
 
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                            how cells adhere to the surface of tissue culture flaask? - basic question (reply: 3)
 
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                            Monocyte culture - How to get the monocytes to adhere to culture plates? (reply: 3)
 
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                            Role of Trypsin in Hep G2 culture -  (reply: 1)
 
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                            Bacteria culture protocols -  (reply: 4)
 
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                            Help for bacteria culture system. -  (reply: 2)
 
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                            is a matrigel layer a 3d cell culture? -  (reply: 6)
 
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                            hemin in cell cultures -  (reply: 8)
 
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                            Hybridoma culture not secreting mouse antibody -  (reply: 6)
 
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                            colonies not growing on plates, but in culture -  (reply: 1)
 
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                            Stem Cell Culture Medium - Stem Cell Culture (reply: 1)
 
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                            culture plate storage - cloning (reply: 2)
 
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                            Media turns Turbid after 3 days of culture -  (reply: 6)
 
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                            Custom plasticware for tissue culture -  (reply: 3)
 
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                            contamination of my culture? -  (reply: 3)
 
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                            Spermatogonia stem cell culture -  (reply: 4)
 
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                            THP-1 cell culture problem: Cells die after thawing -  (reply: 4)
 
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                            Culture flask vent cap -  (reply: 3)
 
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                            Cell culture using laminin -  (reply: 1)
 
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                            bacterial culture -  (reply: 3)
 
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                            Differentiating cultures between commensal and pathogenic -  (reply: 2)
 
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                            is my culture got spoiled? -  (reply: 3)
 
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                            Best Guess:How many cells in Organotypic Slice culture -  (reply: 2)
 
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                            Freshness of a culture for staining purposes -  (reply: 1)
 
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                            How to prepare conditioned media? - Do I have to omit supplements for the culture media? (reply: 2)
 
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                            Mycoplasma - Mycoplasma in mammalian cultures (reply: 7)
 
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                            DMSO in cell culture - is it toxic, does it change signalling? - Citations wellcommed (reply: 2)
 
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                            Force "swimming" cells to adhere - Are there any non-toxic detergents for cell culture? (reply: 4)
 
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                            how to separate dead from viable cells in cultures of non-adherent cells -  (reply: 3)
 
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                            suspension culture of lymphoblastic cells -  (reply: 7)
 
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                            THP-1 cells culture -  (reply: 4)
 
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                            HEK 293 cell culture cytotoxicity? -  (reply: 2)
 
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                            Doesn't anyone use roller bottles to culture cells in anymore? - Let's compare notes. (reply: 2)
 
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                            dendritic cells culture -  (reply: 3)
 
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                            How much antobody to bind to bacterial culture? -  (reply: 1)
 
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                            in vitro model of epilepsy - cell culture (reply: 11)
 
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                            Smooth muscle cell culture -  (reply: 4)
 
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                            how to culture Agrobacterium tumefaciens? -  (reply: 1)
 
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                            Contamination or not? - Cell Culture Contamination (reply: 1)
 
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                            Amplifying cultures - quick question -  (reply: 3)
 
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                            passage of cell culture and protein locolization -  (reply: 1)
 
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                            ChIP assay using Matrigel-3D cell culture -  (reply: 2)
 
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                            culture CD34+ Hematopoietic Stem Cells -  (reply: 2)
 
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                            Preparing cell lysate from cell culture for Western blot -  (reply: 4)
 
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                            How much serum should I put in the tissue culture -  (reply: 5)
 
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                            trouble with primary adult neurosphere cultures -  (reply: 1)
 
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                            human serum in cell culture - is it possible to use human serum in cell culture? (reply: 14)
 
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                            collect secreted proteins in cell culture media -  (reply: 2)
 
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                            Who makes 200-350cm2 tissue culture roller bottles? -  (reply: 8)
 
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                            Embryonic stem cell culture -  (reply: 4)
 
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                            Tissue culturing - Crash down of culture -Unknown reason!! (reply: 4)
 
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                            Macrophage culture -  (reply: 5)
 
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                            How do I culture adherent TZM-bl cells?! -  (reply: 5)
 
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                            can plasma (instead of serum) be used on cell culture? -  (reply: 2)
 
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                            help! cell culture contamination too! -  (reply: 5)
 
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                            reusable cell culture flasks, pipettes etc. -  (reply: 10)
 
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                            Sodium pyruvate in ES cell culture media -  (reply: 2)
 
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                            small intensely fluorescent cells - how to culture them? (reply: 4)
 
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                            microbial contaminant in plant tissue culture...?! -  (reply: 1)
 
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                            Control protein in culture medium for Western? -  (reply: 3)
 
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                            primary colonic epithelial cell culture -  (reply: 1)
 
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                            Cell culture -  (reply: 2)
 
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                            Suspension culture -  (reply: 1)
 
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                            culture cell -  (reply: 1)
 
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                            pyridine, chloroform and metahnol as a solvent for chemopreventive agents - Is there anyone who has used these solvent for cell culture experiment (reply: 6)
 
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                            OPINIONS on Plant Tissue Culture- - Please post your opinions and views on plant tissue culture. (reply: 1)
 
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                            cell culture contamination - after transfection (reply: 15)
 
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                            How do i optimize a shake flask culture conditions for maximum expression of rec -  (reply: 1)
 
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                            culture identification -  (reply: 3)
 
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                            few cells in the cell culture -  (reply: 3)
 
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                            Basic CHO cell culture -  (reply: 3)
 
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                            please help in Anthurium & Orchid Tissue culture - tissue culture medium (reply: 2)
 
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                            Alternative Sera for Cell Culture -  (reply: 6)
 
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                            human dermal fibroblasts tissue culture protocol -  (reply: 2)
 
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                            Cell culture sterility Checks (DMEM) -  (reply: 3)
 
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                            excess penicillin and streptomycin concentration in culture medium? -  (reply: 4)
 
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                            Immunoprecipitation of culture medium -  (reply: 4)
 
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                            Cleaning Campenot Chambers - I´m sttting up DRG cultures in campenot chambers (teflon) and need to (reply: 1)
 
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                            Loss of RNAi after changing culture media? -  (reply: 2)
 
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                            Swine alveolar macrophages culture and PRRSV titer -  (reply: 1)
 
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                            Cell culture pronunciation - pas-SAGE or PAS-sage? (reply: 7)
 
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                            High altitude robotic yeast culture sampling -  (reply: 1)
 
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                            problems about THP-1 cell culture -  (reply: 1)
 
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                            culture contamination...yeast, bacteria, mycoplasma -  (reply: 3)
 
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                            cell culture with serum media and with serum free media and doubts in luciferase -  (reply: 1)
 
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                            Can cell culture media be frozen at -20°C? -  (reply: 6)
 
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                            Can cell culture media be frozen at -20°C -  (reply: 4)
 
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                            any kit or reagent for extracting both DNA and RNA from the same cell culture -  (reply: 3)
 
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                            diluting 2x10e12 culture to 4x10e10 etc? -  (reply: 1)
 
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                            short term & economical preservation of culture -  (reply: 1)
 
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                            what affects cell culture viabilty? -  (reply: 8)
 
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                            Reversibly sticky culture surface -  (reply: 1)
 
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                            Neurosphere culture -  (reply: 2)
 
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                            Cytotoxicity for chondrocyte monolayer culture -  (reply: 5)
 
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                            Stock culture -  (reply: 4)
 
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                            Drosphila S2 cell culture -  (reply: 1)
 
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                            Culture of rat cortical neurons - E16 or E18? (reply: 14)
 
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                            BrdU labeling in primary culture -  (reply: 1)
 
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                            B27/Neurobasal medium for hippocampal neuron culture -  (reply: 7)
 
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                            Culture contamination - yeast contamination (reply: 1)
 
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                            mouse fibroblast culture: what medium? -  (reply: 7)
 
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                            keratinocytes culture procedures -  (reply: 2)
 
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                            Assaying apoptosis on non-detaching cell culture plates - which plates? (reply: 1)
 
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                            preservation of culture - preservation of culture (reply: 2)
 
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                            need help for cord blood mesenchymal stem cell culture -  (reply: 6)
 
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                            Raw 264.7 cells- Culture conditions? -  (reply: 4)
 
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                            Culture based technique -  (reply: 1)
 
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                            FBS and cell culture -  (reply: 9)
 
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                            Help! Large vacuoles observed in HEPG2 cell culture! -  (reply: 3)
 
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                            PC12 cell culture. Do I have to coat plates first? -  (reply: 2)
 
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                            isolation of small RNAs from culture supernatant -  (reply: 11)
 
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                            About the adenovirus infection efficiency on tissue culture -  (reply: 1)
 
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                            how important of Serum proportion of culture medium  in cell culture - SOS (reply: 8)
 
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                            Establishing the hyperlipidimic condition in cell culture -  (reply: 4)
 
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                            E.coli culture and miniprep help -  (reply: 1)
 
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                            estimation of concentration of Ab in hybridoma culture supernatant? -  (reply: 4)
 
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                            Culture medium for MDA-MB-231 / MCF-7 - which media is good enough? (reply: 6)
 
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                            Suspension Cell Culture, Removal of Dead Cells -  (reply: 4)
 
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                            cell culture - a beginner -  (reply: 3)
 
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                            Suspension cell culture. -  (reply: 5)
 
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                            Measuring proliferation via cell culture medium - is it possible? (reply: 2)
 
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                            fungal contamination - fungal in cell culture (reply: 4)
 
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                            Overnight culture methods -  (reply: 5)
 
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                            cell culture treatment, drug testing -  (reply: 2)
 
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                            Confocal microscopy with primary cell cultures -  (reply: 8)
 
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                            Serum free hippocampal slice cultures -  (reply: 4)
 
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                            Subcultures of primary cultures - would you use them for your experiments? -  (reply: 3)
 
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                            ELISA Question - Finding the concentration of Ab from cell culture (reply: 4)
 
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                            How to maintain pH of designed culture media? - Autoclaving, metabolism of cells cause changes in pH (reply: 2)
 
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                            Porcine alveolar macrophages culture -  (reply: 2)
 
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                            lymphocyte culture - problem in isolating lymphocytes from spleen culture (reply: 1)
 
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                            Cell culture contamination, antibiotic recommendation, any recommd on Normocin -  (reply: 7)
 
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                            Culture Confluency -  (reply: 4)
 
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                            cell culture contamination -  (reply: 2)
 
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                            Remove BSA from cell culture medium -  (reply: 5)
 
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                            Establishment of primary fibroblast culture -  (reply: 2)
 
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                            Establishment of primary fibroblast culture -  (reply: 3)
 
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                            Help! neural stem cells culture - no neurosphere after P1 - Please troubleshoot my protocol (reply: 2)
 
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                            Colony PCR or PCR with Growing culture in Broth - Trying to detect the cloned fragment in living cells (reply: 16)
 
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                            Primary cultures of breast carcinoma for FACS -  (reply: 1)
 
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                            Basics of Cell Culture -  (reply: 2)
 
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                            Selection of cell culture media - for HT29 colon cancer cells (reply: 2)
 
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                            Primary Myoblast Culture - Could u help me in myoblast culture and differention (reply: 1)
 
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                            selection of culture medium -  (reply: 1)
 
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                            Filtration of bacterial culture -  (reply: 1)
 
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                            can arginine be used as nitric oxide donor compound in cell culture. - kinly help!!!!!!!! (reply: 3)
 
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                            rat1 cells freezing and thawing - how to culture rat1 fibroblasts (reply: 1)
 
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                            Contamination rate high! - tissue culture (reply: 4)
 
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                            Trouble going from plates to liquid culture -  (reply: 2)
 
- 
                            cell culture -  (reply: 4)
 
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                            Higher biomass yield in batch cultures -  (reply: 1)
 
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                            his-tag protein expression - inoculation of large cultures with plate colonies/ starter cultures vs. glycerol (reply: 1)
 
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                            fiborblast contamination in primary culture -  (reply: 7)
 
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                            banana culture -  (reply: 1)
 
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                            Cell Culture Book - any suggestion? (reply: 29)
 
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                            Culture of normal human mammary epithelial cells -  (reply: 2)
 
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                            cell culture -  (reply: 2)
 
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                            WB with cell culture medium -  (reply: 2)
 
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                            Thp-1 cell culture problems -  (reply: 5)
 
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                            serum free cell culture medium -  (reply: 6)
 
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                            Cell Culture with Human Plasma -  (reply: 2)
 
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                            good protocol for HeLA cells culture -  (reply: 3)
 
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                            rat's umbilical cord culture -  (reply: 4)
 
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                            Protocol for protein isolation from culture media -  (reply: 3)
 
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                            How to limit fibroblast cell contamination in epithelial cell culture -  (reply: 9)
 
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                            How to culture and assay Bacteriophages? -  (reply: 1)
 
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                            can antibodies be added to cell culture for 10d before stain - immunocytochemistry (reply: 1)
 
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                            Overlay cell culture medium with oil against evaporation - which oil? (reply: 2)
 
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                            culture blood vessel vitro -  (reply: 2)
 
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                            CHO culture: what media and additives? - for stable Fc fusion protein expression (reply: 4)
 
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                            cell culture western blot -  (reply: 15)
 
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                            How can i decontaminated my cell culture ? -  (reply: 4)
 
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                            Can adherent culture be transformed into suspension cells? -  (reply: 4)
 
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                            effects  of CO2 level on mammalian cell culture -  (reply: 3)
 
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                            human bone marrow msc culture -  (reply: 2)
 
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                            Is cell culture considered in vivo? -  (reply: 4)
 
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                            E.Coli culture contamination 2 -  (reply: 10)
 
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                            Need to Slow down Cell Culture growth -  (reply: 2)
 
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                            Tetracycline in cell culture media -  (reply: 5)
 
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                            plate culture of Streptococcus pyogenes -  (reply: 2)
 
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                            Loss of cell viability in primary culture -  (reply: 4)
 
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                            What s the maximum allowable limit of DMSO as a solvent in cell culture -  (reply: 8)
 
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                            Question on bacterial culture -  (reply: 5)
 
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                            PC12 culture -  (reply: 6)
 
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                            Fibroblats contamination in primary cell cultures - Fibroblats contamination in primary cell cultures (reply: 6)
 
- 
                            Help with HeLa cells culture... -  (reply: 10)
 
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                            Why tumor cells are hard to culture -  (reply: 3)
 
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                            contamination of plant tissue culture -  (reply: 2)
 
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                            T cell culture - ag specific T cell culture (reply: 1)
 
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                            T cell culture -  (reply: 2)
 
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                            What's difference between RPMI and DMEM - which media I should use to culture cells after transfection? (reply: 3)
 
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                            E.coli culture contamination -  (reply: 6)
 
- 
                            Immunofluorescence of frozen cell culture inserts -  (reply: 6)
 
- 
                            mold problems in cell culture -  (reply: 1)
 
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                            How to avoid contamination in tissue culture? -  (reply: 11)
 
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                            Tissue Culture of Sealing Red Wax Palm -  (reply: 3)
 
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                            primary cell culture death -  (reply: 8)
 
- 
                            anyone worked with fish tissue culture? -  (reply: 1)
 
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                            ELISA IL12 p70 from mouse dendritic cells culture SN -  (reply: 2)
 
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                            Primary hippocampal cultures -  (reply: 5)
 
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                            new lab, cells die after passaging - general fish cell culture methods (reply: 5)
 
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                            Any effect of different culture dishe size on cell? -  (reply: 20)
 
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                            mouse spleen cell culture -  (reply: 2)
 
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                            how long to culture the bacteria after electroporation -  (reply: 10)
 
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                            Has any one successfully decontaminated a contaminated cell culture -  (reply: 3)
 
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                            Cholera toxin in culture medium - Effect of cholera toxin (reply: 1)
 
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                            culture of synovial fibroblasts (mouse) -  (reply: 1)
 
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                            transfection of rat spinal cord neuronal cultures -  (reply: 3)
 
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                            Why HepG2 and HUH7 - for hepatitis cell culture matter. (reply: 3)
 
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                            tissue n cell culture -  (reply: 3)
 
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                            cell culture contamination -  (reply: 2)
 
- 
                            Question on Overgrown Cell Culture -  (reply: 9)
 
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                            Differential media neuronal culture -  (reply: 1)
 
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                            Triglycerides in culture medium -  (reply: 2)
 
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                            Culture of adherent SH-Sy5y Neuroblastoma Cell Line -  (reply: 5)
 
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                            E coli culture -  (reply: 3)
 
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                            Dissociated neuronal cultures -  (reply: 2)
 
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                            Cell culture Contamination - Again! (reply: 13)
 
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                            microglia cell culture -  (reply: 5)
 
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                            PC12 cell culture -  (reply: 5)
 
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                            Setting up a Cytoxicity Assay - cell culture (reply: 1)
 
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                            yeast contamination in my cell culture -  (reply: 11)
 
- 
                            Cell Biologists working in 3d cell cultures -  (reply: 1)
 
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                            problem with culture revival & passage -  (reply: 1)
 
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                            Use of antibiotics in cell culture? -  (reply: 5)
 
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                            Impact of dead cells in a culture? - When cells die, how toxic can they become to viable cells? (reply: 4)
 
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                            Is myc endogenously expressed in primary hippocampal neuronal cultures? - positive myc immunostaining in hippocampal cultures (reply: 1)
 
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                            how to solve the problem cell culture contamination? -  (reply: 2)
 
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                            Aspergillus niger culture media -  (reply: 3)
 
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                            Tissue Culture -  (reply: 1)
 
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                            Good book on cell culture -  (reply: 5)
 
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                            Culture Contamination (MCF7) -  (reply: 4)
 
- 
                            macrophages culture, please help -  (reply: 1)
 
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                            Protein didn't express - why? - M15 E. coli culture and pQE30UA vector; pTrcHis2TOPO and TOP10 E. coli cells (reply: 10)
 
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                            Unknown culture contamination? -  (reply: 7)
 
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                            Fetal bovine serum and cell culture -  (reply: 5)
 
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                            Maize tissue culture -  (reply: 5)
 
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                            explant culture! -  (reply: 4)
 
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                            What's the maximum amount of DMSO you can use in cell culture? -  (reply: 4)
 
- 
                            How long do primary monocytes/macrophages last in culture? -  (reply: 2)
 
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                            desert plants - for tissue culture (reply: 6)
 
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                            which antibiotic for primary culture? -  (reply: 3)
 
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                            about culture temperature -  (reply: 5)
 
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                            methods of inducing stress in cell culture -  (reply: 8)
 
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                            probles when culture 293T culture? -  (reply: 6)
 
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                            Bacterial culture -  (reply: 1)
 
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                            getting rid of shaky hands during cell culture/pipetting -  (reply: 9)
 
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                            How often do you change culture vessels? -  (reply: 12)
 
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                            somaclonal variation in tissue culture -  (reply: 4)
 
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                            Why use fetal calf serum for cell culture? - Why not use Fetal pig serum? (reply: 15)
 
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                            Splenocyte culture - Cell death upon in vitro culture (reply: 1)
 
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                            Tissue culture Infective dose - Infect the Human tumor ell culture by Newcastle virus (reply: 1)
 
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                            When air bubbles is not encouraged in tissue culture? -  (reply: 4)
 
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                            Cell Culture Microscope - Microscopes (reply: 4)
 
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                            arabidopsis leaf tissue culture -  (reply: 3)
 
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                            virus culture and harvesting -  (reply: 1)
 
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                            why overnight culture is necessary -  (reply: 3)
 
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                            How to culture stable cell lines? -  (reply: 3)
 
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                            Primary culture hepatocytes of mouse -  (reply: 2)
 
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                            Persistent cell culture contamination by fungi and bacteria - Cell culture, cell biology (reply: 6)
 
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                            possible contamination of a cell culture? -  (reply: 3)
 
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                            how do initiation alpinia galanga (plant culture)? - plant biotechnology (reply: 1)
 
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                            Cornea Epithelial Cell culture -  (reply: 1)
 
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                            culture time for maxi prep -  (reply: 3)
 
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                            Plant tissue culture -  (reply: 1)
 
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                            TCID50 - Tissue culture infective dose of virus. (reply: 1)
 
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                            questions about bacteria culture medium -  (reply: 1)
 
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                            reasons for apoptosis in primary cell culture? -  (reply: 2)
 
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                            Plant tissue culture -  (reply: 7)
 
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                            help!-- plant cell culture -  (reply: 1)
 
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                            how to culture primary neuron in coated coverslip - how to culture primary neuron in coated coverslip (reply: 5)
 
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                            extract peptides from tissue/cell culture for ELISA kits -  (reply: 3)
 
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                            Will the addition of CO2 to cultures boost cyanobacterial growth rate? -  (reply: 2)
 
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                            Plant tissue culture -  (reply: 4)
 
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                            general cell culture (and IMR90 line) - any resource to find out info about lines (reply: 1)
 
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                            What type of cell culture contamination is this? - large pieces of jelly-like material!! (reply: 7)
 
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                            Re-innoculate overnight culture for miniprep -  (reply: 4)
 
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                            Trouble with Mycobacterium marinum!? - Microbiology: bacterial culture (reply: 2)
 
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                            Isolation and culture of white blood cells. -  (reply: 1)
 
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                            Neuro2A cell culture Death and Debris - Problem keeping cells alive for more than a week. (reply: 2)
 
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                            Paraformaldehyde detaches my 3T3 culture -  (reply: 2)
 
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                            Qustions about stem cell culture -  (reply: 1)
 
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                            need help for  culture protacal of new stem cell -  (reply: 5)
 
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                            A few questions about autoclaving culture medium -  (reply: 11)
 
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                            Where to Obtain Pure Algal Cultures -  (reply: 3)
 
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                            Hippocampus Neuron Primary Culture -  (reply: 1)
 
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                            puromycin in culture medium -  (reply: 3)
 
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                            Moving Dots in cell culture - Contamination? -  (reply: 7)
 
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                            Growing bacteria for several cultures -  (reply: 6)
 
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                            Drosophila cell culture -  (reply: 3)
 
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                            Culture problems of CRL-2331 cells (ATCC) -  (reply: 3)
 
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                            Cardiac stem cells - Isolation and Culture (reply: 2)
 
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                            Problems with Ba/F3 cell culture -  (reply: 1)
 
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                            help: Drosophila cell culture -  (reply: 1)
 
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                            Water or TSB to dilute an over night culture!? -  (reply: 6)
 
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                            How to isolate and culture murine epidermal langerhans cells -  (reply: 2)
 
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                            Primary cell culture: labelling in vivo -  How to label a protein for Fluorescence Microscopy? (reply: 2)
 
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                            Primary cell culture: labelling in vivo - How to label a protein for Fluorescence Microscopy (reply: 3)
 
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                            culture maintainence - bacterial culture maintainence (reply: 1)
 
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                            tissue culture about zizyphs jujuba - please help me! (reply: 2)
 
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                            How to culture rat dorsal root ganglia cell? -  (reply: 1)
 
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                            Shipping cells in suspension culture -  (reply: 6)
 
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                            tissue culture Garlic/Onion - protocol (reply: 4)
 
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                            Primary Culture contamination issues -  (reply: 2)
 
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                            Primary culture of breast tumour - Does anyone know the tissue slice method? (reply: 3)
 
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                            Help me - vero-MCF-7 Hela cells culture (reply: 1)
 
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                            Primary culture - keratinocytes - how do I know? (reply: 6)
 
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                            coat culture dish for HEK-293T cells? -  (reply: 5)
 
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                            Cytokine/chemokine detection in culture supernatant -  (reply: 6)
 
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                            Mycoplasma Testing in culture medium -  (reply: 2)
 
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                            keratinocyte culture media -  (reply: 8)
 
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                            change in cell morphology in culture - cell culture (reply: 2)
 
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                            Help with endothelial cells - Endothelial Cells Culture (reply: 4)
 
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                            PI and human lymphocyte cultures -  (reply: 1)
 
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                            DRG cell culture -  (reply: 6)
 
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                            Add L-glutamine in cell culture medium? -  (reply: 5)
 
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                            culture media left out for too long? -  (reply: 7)
 
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                            HOW to culture  bone marrow monoclear cells(BMMNCs)? -  (reply: 6)
 
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                            Serial dilution and quantitative culture of S. aureus -  (reply: 4)
 
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                            Fibroblast and epithelial cells in tumor samples and primary cultures -  (reply: 2)
 
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                            lncap cell culture -  (reply: 3)
 
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                            how do you maintain a culture of N. gonorrhea? what type of media? -  (reply: 2)
 
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                            collagen-coated culture dishes - How to make? (reply: 14)
 
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                            Cell culture contaminations -  (reply: 6)
 
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                            mouse embryonic cortical organotypic cultures -  (reply: 1)
 
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                            help with smooth muscle cell culture -  (reply: 4)
 
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                            HL60 in culture and differentiation -  (reply: 1)
 
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                            Tissue culture -Tips -  (reply: 6)
 
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                            pH balance - HCO3/CO2 buffer in culture media (reply: 1)
 
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                            equine fibroblast culture -  (reply: 2)
 
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                            Western blots on contaminated cell cultures? - Mycoplasma (reply: 1)
 
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                            Culture medium for MCF-10A -  (reply: 4)
 
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                            Sphere cells suspension culture -  (reply: 2)
 
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                            Mouse ES cell culture - ES cell culture for the purpose of gene targeting (reply: 3)
 
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                            HeLa in suspension culture - I want to keep HeLa cells in suspension culture (reply: 2)
 
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                            cho-k1 culture -  (reply: 1)
 
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                            30 degrees versus 37 degrees for E coli culture -  (reply: 7)
 
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                            Glycyrrhizin (GL) and cell culture -  (reply: 1)
 
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                            Websites or books about stem cell culture -  (reply: 2)
 
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                            primary culture transfection problems - any transfection methods on primary granulosa cell (reply: 1)
 
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                            Help with concentrating proteins in culture medium -  (reply: 4)
 
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                            bacterial culture growth on plate but not in liquid -  (reply: 3)
 
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                            primary culture - measuring transcription - mRNA expression in primary culture (reply: 1)
 
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                            why does DMEM pH increase - Tissue culture (reply: 2)
 
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                            Adenovirus culture/isolation -  (reply: 3)
 
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                            contamination of cell culture by another cell type -  (reply: 1)
 
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                            help:how to culture mouse embryonic neuron(E13.5) ? -  (reply: 2)
 
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                            Minimal Media Recipes - for Fed-Batch Cultures of EColi (reply: 1)
 
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                            Organ Culture -  (reply: 4)
 
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                            mouse aortic smooth muscle cell isolation - cell culture (reply: 1)
 
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                            questions about mass spectrometry and cell culture -  (reply: 1)
 
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                            Primary Cell Culture - Tips for PHK culture (reply: 2)
 
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                            Separation of dead cells from cultures -  (reply: 8)
 
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                            plant tissue culture protocols - basic plant tissue culture techniques for students (reply: 8)
 
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                            Function of beta-mercaptoethanol in cell culture medium -  (reply: 6)
 
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                            Prinamry rat neuronal culture -  (reply: 3)
 
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                            Black Debris in T-Cells - debris in my cell culture (reply: 3)
 
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                            Mold Contamination in cell culture - Mold and Cell Culture (reply: 7)
 
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                            dendritic cell isolation - cell culture (reply: 1)
 
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                            what to add to media - cell culture medium (reply: 7)
 
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                            Tissue culture of algea -  (reply: 3)
 
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                            spinner flasks - cell culture (reply: 1)
 
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                            Primary Culture of keratinocytes - How to start a keratinocyte culture (reply: 4)
 
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                            best method for detaching cells - in culture (reply: 4)
 
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                            Keratinocyte cultures with serum or serum free media -  (reply: 1)
 
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                            can PBMC proliferate without mitogen??? - Isolate and culture the PBMC with mitogen (reply: 2)
 
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                            Fixation of cell culture with Formaldehyde - need protocol (reply: 3)
 
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                            fibroblast contamination - cell culture (reply: 2)
 
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                            Preparing cultures of know concentration - How to do it? (reply: 5)
 
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                            Side-effects of 5-aza - non-specific demethylation in primary culture (reply: 2)
 
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                            Concentration of secreted protein in culture media - Concentration of culture media (reply: 7)
 
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                            how can i culture virus in primary cell lines? - microbiology (reply: 1)
 
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                            coatings for growing cells on glass coverslips - tissue culture and confocal microscopy (reply: 4)
 
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                            Reusing old culture flasks? -  (reply: 1)
 
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                            Macrophage culture problems! please help! -  macrophages (reply: 1)
 
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                            Is TE buffer toxic to cultured cells? - cell culture (reply: 4)
 
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                            detecting Mycoplasma in cultures -  (reply: 7)
 
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                            help: about slice culture - about slice culture (reply: 1)
 
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                            Do you reuse cell culture vessels? -  (reply: 3)
 
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                            more help: cell culture problem -  (reply: 1)
 
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                            100mm culture dish -  (reply: 5)
 
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                            CO3 / HCO3 content of the culture medium -  (reply: 3)
 
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                            overnight culture -  (reply: 3)
 
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                            Fungal Infection in mesangial cells - Primary Cell Culture (reply: 2)
 
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                            Ingredient for keratinocyte culture medium -  (reply: 1)
 
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                            Cellular Responses to insulin and growth factor - Cell Culture (reply: 2)
 
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                            Shaker rotation to grow bacterial cultures? -  (reply: 8)
 
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                            "Cell culture discard in chloros (10,000) ppm" ? -  (reply: 4)
 
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                            human rTGFß for mouse cell culture applications? -  (reply: 1)
 
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                            Scaling up from a little bacterial culture? -  (reply: 1)
 
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                            50% stock cultures -  (reply: 2)
 
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                            does fungi swim or move? - cell culture contamination (reply: 8)
 
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                            Tissue culture and cell culture? -  (reply: 4)
 
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                            Cell culture contamination - small black adherent dots!! (reply: 4)
 
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                            Ventation of culture vessles -  (reply: 5)
 
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                            Antibiotic use in Pichia cultures -  (reply: 1)
 
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                            MDA-MB-231 cell culture -  (reply: 7)
 
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                            bacterial culture contamination -  (reply: 1)
 
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                            Filtration of primary cell culture, how?? -  (reply: 2)
 
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                            breast primary culture cells -  (reply: 2)
 
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                            filtering for cell culture stuff - filter cell culture (reply: 5)
 
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                            C. elegans culture -  (reply: 2)
 
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                            tgf-beta in cell culture -  (reply: 1)
 
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                            How to culture T cells -  (reply: 1)
 
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                            Mouse pancreatic primary culture: protocol is needed -  (reply: 4)
 
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                            mouse bone marrow culture - mesenchymal stem cell - Cell culture (reply: 2)
 
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                            Extraction of adenovirus DNA from cell culture for PCR - protocol (reply: 2)
 
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                            breast cancer cell primary culture -  (reply: 2)
 
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                            primary keratinocytes - HeKn - problem with culture (reply: 2)
 
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                            Bringing tumor line from animal back to cell culture -  (reply: 2)
 
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                            Sterilizing a Cell Culture Room - We had a flood + mold, where do I start? (reply: 1)
 
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                            cancer cell culture - protocols on cancer cell culture (reply: 8)
 
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                            E. coli culture contamination -  (reply: 2)
 
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                            fibroblast contamination in primary culture -  (reply: 1)
 
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                            Contamination issue in cell culture -  (reply: 109)
 
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                            LCL B cell culture - Death within 24 hours of thawing - LCL b cells are dying within 24 hours of thawing. (reply: 3)
 
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                            non-enzymatic dissociation - cell culture (reply: 1)
 
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                            Problems with B-cell line BJAB - clumps formed by cells in culture (reply: 1)
 
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                            Freezer culture fails to retain plasmid -  (reply: 3)
 
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                            Equipment requirements to culture cells - information needed on kidney cells (reply: 2)
 
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                            Ht-29 Subculture - Cell culture (reply: 1)
 
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                            About R28 cells culture -  (reply: 2)
 
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                            tissue culture of jatropha - tissue culture of biodiesel producing plant (reply: 22)
 
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                            mycoplasma and fungus - tissue culture infection (reply: 2)
 
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                            preventing infection of cell cultures -  (reply: 3)
 
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                            Plasmid extraction from E.coli culture -  (reply: 7)
 
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                            anaerobic bacteria - how to stock culture (reply: 2)
 
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                            cell culture RNA extraction - cell culture RNA extraction (reply: 4)
 
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                            help: primary endothelial cell culture! -  (reply: 3)
 
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                            tissue culture floor cleaning - standard protocol?? (reply: 1)
 
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                            Myofibroblast Culture -  (reply: 1)
 
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                            mouse microglia - cell culture and primary (reply: 4)
 
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                            concentrate protein in culture media? -  (reply: 2)
 
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                            Why we use high glucose concentration in culture? -  (reply: 1)
 
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                            4 layers instead of a monolayer in cell culture -  (reply: 2)
 
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                            Cell culture problem - weird stuff grow in my cells -  (reply: 6)
 
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                            Plant Culture Cell -  (reply: 1)
 
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                            HEK293 cell culture protocol -  (reply: 1)
 
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                            Serum in Cell Culture - Use of FBS etc. in cell culture (reply: 3)
 
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                            Shipping Cell Cultures - Shipping Cell Cultures (reply: 3)
 
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                            Monocyte culture -  (reply: 5)
 
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                            using culture sup of one cell line  to induce 2nd cell line -  (reply: 1)
 
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                            how to culture a cell line of "fibroblast" -  (reply: 5)
 
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                            Shipping primary neuronal cultures o/n - How do I shipprimary DRG's overnight (reply: 1)
 
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                            Yeast in Cell Culture... - How to erase the Yeast? (reply: 2)
 
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                            cells washed away - cell culture problems (reply: 6)
 
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                            Endothelial cells culture - Protocols for the culture of endothelial cells, special tips (reply: 2)
 
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                            seperation of epithelial cells and fibroblast from a biopsie - a culture of epithelial cells is needed without fibroblasts (reply: 1)
 
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                            Formalin fixation¶ffin-embedding of tissue culture cells - Formalin fixation¶ffin-embedding of tissue culture cells (reply: 1)
 
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                            how to culture human monocytes... - advice please (reply: 2)
 
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                            animal cell culture -  (reply: 2)
 
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                            How to culture macrophages from human blood? - macrophages (reply: 1)
 
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                            Endotoxin in culture media - Endotoxin in culture media (reply: 2)
 
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                            spleenocytes die in culture - spleenocytes die in culture when put for invitro stimulation (reply: 2)
 
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                            Macrophage culture -  (reply: 4)
 
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                            Pituitary cell culture -  (reply: 1)
 
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                            How to concentrate protein from culture medium - for Western blot (reply: 1)
 
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                            PCR and Cultures - PCR and Cultures (reply: 1)
 
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                            lymphocytes - help wanted for culture (reply: 1)