Hello everyone!
do somenone knows a protocol on how to prepare primary dissociated neuronal cultures of striatum or SN? I currently have experience on cortical and hippocampal cultures from P0-P2 rats, so it would be better to me to use postnatal brains instead of embryonic. I've found a lot of protocol on E15-E19 rats but almost no-one on P0-P2... why is that? Dopaminergic neuron doesn't grow from this stage?
Thanks a lot for all the help and suggestion

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see if result no. 6 is helpful:

http://search.vadlo.com/b/q?sn=158621799&a...tures&rel=0

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Thanks cellcounter...! seems quite complicated...in your experience...the astrocyte cell layer (feeder) could be eliminated if the cells are grown in Neurobasal-B27? (as in cortex/hippocampus)
thanks again

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