Culture based technique - (Apr/13/2007 )
I am trying to culture bacterial colonies from soil which is procured from a depth of 45 m. At this depth the microbial diveristy is expected to be very very less. However when i used the soil extract to plate got 6 colonies. How can i ascertain whether the colonies are contaminants or bacteria from the soil?
If you can, do the experiment two more times. best 2/3 wins.