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Culture media with human serum gives false positive - (Jun/27/2008 )

Hi! I'm using a MIP-4 ELISA to detect the protein in cell culture supernatant, but I have the problem that my wells with cell culture media alone give me a high positive reading . My medium contains human serum and the company (Biosource) told me the problem was probably the serum. Is there anything I can do to still be able to read my samples??


Dilute the samples the serum interferant may dilute out more quickly then the corresponding antigen.