Monocyte culture - (Mar/27/2001 )
I want to grow sheep monocytes obteined from blood, but I don't know very well which should be the composition of the growing medium. Can anybody help me with this? Another question, this cell type needs CO2? Thank you.
I grow promonocyte derived or related cell lines (U937, THP1, HL60). We use RPMI 1640 + 10% FBS (and yes, 5% CO2).For growing primary monocytes or lymphocytes additionof IL-2 will prolong survival.
Dear Sir/MadamI am Ph.D. Student in Medicine and my project is designed to work mostly with human monocytes and macrophagesFor your work you need RPMI1640 and 10% HI FCS and 10% HI AB HS, and also you need C02 incubatorFor more information please email me to get more related information to your work.Good Luck
Mohammed AlaliUniversity of SydneyNSW Australia
can anyone tell me how to harvest monocytes from cell culture wells without damaging them-can they just be simply scraped? If they're apoptosing (as we are looking at apoptosis)-should we also be analysing the supernatant after harvesting also i.e are they likely to detachfrom the well surface?