rat's umbilical cord culture - (Nov/05/2006 )
I want to start to culture rats umbilical cord, but I don't know how to do it. is there anybody experienced in culturing the umbilical cord? can share information with me...
aha, I have cultured umbilical cord mesenchymal cells for a long time. If you have some questions, I will be glad to discuss them with you.
Are you going to culture rat umbilical cord endothelai cells? If yes, you can use collagenase.
From what species did you culture these cells? Did you also culture mesenchymal stem cells from bone?
Until now I only isolated MSCs from bone. Next week I may get umbilical cords for collecting cells. The method seems to be the same, but maybe you know some important things I have to keep in mind.
I'm now using the human MSC-derived from umbilical cord blood, and I want to add the in vivo experiment.
Could you tell me the method culturing MSC in rat or mouse??