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Culture medium for MCF-10A - (Sep/13/2005 )

I will buy MCF 10A cell line from ATCC, and I wonder what type of cultre medium I should use. I read specification from ATCC but for me it seems to be a little strange. What do you think about it? What kinde of medium you usually use, please help me!!!!

-augustyna-

MCF10A culture medium:

DMEM/F12 with 15 mM hepes buffer
5% horse serum
10 ug/ml insulin
20 ng/ml EGF
100 ng/ml choleratoxin
0.5 ug/ml hydrocortisone
pen/strep

-Theo22-

And the reference:
Soule et al (1990) Cancer Res 50, p6070-6086

-Theo22-

we omit the cholera toxin and they seem to grow fine

-vw3sarah22-

Thanks a lot smile.gif

-augustyna-